Anti-HDAC2 antibody - C-terminal (ab227149)
Key features and details
- Rabbit polyclonal to HDAC2 - C-terminal
- Suitable for: ChIP, IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC2 antibody - C-terminal
See all HDAC2 primary antibodies -
Description
Rabbit polyclonal to HDAC2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human HDAC2 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q92769 -
Positive control
- ICC/IF: HeLa cells. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; C2C12 and Rat2 whole cell lysates. IP: HeLa whole cell lysate. ChIP: HEK-293T whole cell lysate/extract. IHC-P: Cal27 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ChIP |
Use at an assay dependent concentration.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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Notes |
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ChIP
Use at an assay dependent concentration. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. -
Tissue specificity
Widely expressed; lower levels in brain and lung. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsS-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3066 Human
- Entrez Gene: 15182 Mouse
- Entrez Gene: 84577 Rat
- Omim: 605164 Human
- SwissProt: Q92769 Human
- SwissProt: P70288 Mouse
- Unigene: 3352 Human
- Unigene: 19806 Mouse
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Alternative names
- D10Wsu179e antibody
- HD 2 antibody
- HD2 antibody
see all
Images
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All lanes : Anti-HDAC2 antibody - C-terminal (ab227149) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extracts
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 4 : HepG2 (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
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ab227149 immunoprecipitates HDAC2 protein-DNA complex in ChIP experiments.
ChIP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract.
A: 5 μg preimmune rabbit IgG
B: 5 μg of ab227149
The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter. -
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HDAC2 (green) using ab227149 at 1/500 dilution in ICC/IF. Alpha-tubulin filaments were labeled (red).
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Paraffin embedded Cal27 (human) xenograft tissue stained for HDAC2 using ab227149 at 1/100 dilution in immunohistochemical analysis.
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HDAC2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate. Western blot was performed from the immunoprecipitate using ab227149 at 1/1000 dilution. Anti-rabbit IgG was used as a secondary reagent.
Lane 1: 40 μg HeLa whole cell lysate.
Lane 2: Control with 2 μg of preimmune rabbit IgG.
Lane 3: Immunoprecipitation of HDAC2 protein by 2 μg of ab227149.
7.5% SDS-PAGE gel.
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Anti-HDAC2 antibody - C-terminal (ab227149) at 1/1000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
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Anti-HDAC2 antibody - C-terminal (ab227149) at 1/1000 dilution + C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227149 has been referenced in 1 publication.
- Mattioli E et al. Altered modulation of lamin A/C-HDAC2 interaction and p21 expression during oxidative stress response in HGPS. Aging Cell 17:e12824 (2018). PubMed: 30109767