Please note product formulation is not optimised for ChIP application
Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
Tissue specificityWidely expressed; lower levels in brain and lung.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational modificationsS-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
Western blot - Anti-HDAC2 antibody [EPR5001] (ab124974)
All lanes : Anti-HDAC2 antibody [EPR5001] (ab124974) at 1/10000 dilution
Lane 1 : HeLa cell lysate Lane 2 : SH-SY5Y cell lysate Lane 3 : Jurkat cell lysate Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 55 kDa
ChIP - Anti-HDAC2 antibody [EPR5001] (ab124974)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 10µg of ab124974 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.
References for Anti-HDAC2 antibody [EPR5001] (ab124974)
This product has been referenced in:
Lin MY et al. Redirection of Epithelial Immune Responses by Short-Chain Fatty Acids through Inhibition of Histone Deacetylases. Front Immunol6:554 (2015).
Read more (PubMed: 26579129) »