Anti-HDAC3 antibody [3G6] - C-terminal (ab170199)

Overview

  • Product name
    Anti-HDAC3 antibody [3G6] - C-terminal
    See all HDAC3 primary antibodies
  • Description
    Mouse monoclonal [3G6] to HDAC3 - C-terminal
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human HDAC3 aa 411-428 (C terminal).
    Sequence:

    NEFYDGDHDNDKESDVEI

  • Positive control
    • mouse NIH 3T3 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab170199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/800. Predicted molecular weight: 48 kDa.
IP Use at an assay dependent concentration.

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated in vitro.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

Images

  • Lane 1 : Anti-HDAC3 antibody [3G6] - C-terminal (ab170199) at 1/100 dilution
    Lane 2 : Anti-HDAC3 antibody [3G6] - C-terminal (ab170199) at 1/200 dilution
    Lane 3 : Anti-HDAC3 antibody [3G6] - C-terminal (ab170199) at 1/400 dilution

    All lanes : NIH 3T3 cell lysate

    Predicted band size: 48 kDa


    Exposure time: 30 seconds


    NIH 3T3 cell lysate was separated by 10% SDS PAGE and blotted onto nitrocellulose membrane. The membrane was blocked in 3% NFDM in PBS-T for 1h at RT and incubated at 4°C O/N with ab170199.

References

ab170199 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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