Overview

  • Product nameAnti-HDAC3 antibody
    See all HDAC3 primary antibodies
  • Description
    Rabbit polyclonal to HDAC3
  • SpecificityDetects endogenous levels of total HDAC3 protein.
  • Tested applicationsSuitable for: ELISA, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from C-terminal of human HDAC3

  • Positive control
    • COS7 cells, extracts from HepG2 cells, human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab61216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated in vitro.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

Anti-HDAC3 antibody images

  • All lanes : Anti-HDAC3 antibody (ab61216) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells
    Lane 2 : extracts from HepG2 cells with immunizing peptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • Ab61216 at 1/50-1/100 dilution staining human breast carcinoma tissue without (left) and with (right) immunizing peptide; paraffin embedded.
  • Ab61216 at 1/500-1/1000 dilution staining COS7 cells without (left) and with (right) immunizing peptide.

References for Anti-HDAC3 antibody (ab61216)

This product has been referenced in:
  • Pancione M  et al. Right-sided rhabdoid colorectal tumors might be related to the serrated pathway. Diagn Pathol 8:31 (2013). IHC-P ; Human . Read more (PubMed: 23425390) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab61216.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"