Overview

  • Product nameAnti-HDAC3 antibody
    See all HDAC3 primary antibodies
  • Description
    Rabbit polyclonal to HDAC3
  • Tested applicationsSuitable for: Sandwich ELISA, ELISA, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human HDAC3 around the phosphorylation site of serine 424.

  • Positive control
    • Human skin tissue. Extracts from NIH/3T3 cells. HeLa cells, treated with TNF-a.

Properties

Applications

Our Abpromise guarantee covers the use of ab63353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [7G6C5] to HDAC3 (ab76359) as Capture.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated in vitro.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

Anti-HDAC3 antibody images

  • Standard Curve for HDAC3 (Analyte: HDAC3 protein (Tagged) (ab82071)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [7G6C5] to HDAC3 (ab76359) at 5 ug/ml and Detector Antibody Rabbit polyclonal to HDAC3 (ab63353) at 0.5 ug/ml.
  • All lanes : Anti-HDAC3 antibody (ab63353) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3 cells, without immunizing peptide
    Lane 2 : extracts from NIH/3T3 cells, with immunizing peptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of HDAC3 expression in paraffin-embedded human skin tissue, using ab63353 at 1/50 dilution. Right: sample treated with immunising peptide. Left: untreated sample.
  • Immunofluorescence analysis of HDAC3 expression in HeLa cells, using ab63353 at 1/500 dilution. Left: Cells treated with TNF-a (20nM, 15mins). Right: Untreated cells.

Protocols

References for Anti-HDAC3 antibody (ab63353)

This product has been referenced in:
  • Razidlo DF  et al. Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat. PLoS One 5:e11492 (2010). WB ; Mouse . Read more (PubMed: 20628553) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"