Overview

  • Product nameAnti-HDAC3 antibody - ChIP Grade
    See all HDAC3 primary antibodies
  • Description
    Rabbit polyclonal to HDAC3 - ChIP Grade
  • Tested applicationsSuitable for: IP, ChIP, WBmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    AKTVAYFYDPDVGNFH

    , corresponding to N terminal amino acids 2-17 of Human HDAC3

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab47237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution. Refer to Caretti et al (2003, Fig.3) and Imbriano (2005, Fig 7) for details.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).

Target

Anti-HDAC3 antibody - ChIP Grade images

  • Anti-HDAC3 antibody - ChIP Grade (ab47237) at 2 µg/ml + HeLa cell lysate

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • All lanes : Anti-HDAC3 antibody - ChIP Grade (ab47237) at 2 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

References for Anti-HDAC3 antibody - ChIP Grade (ab47237)

This product has been referenced in:
  • Doyle K & Fitzpatrick FA Redox signaling, alkylation (carbonylation) of conserved cysteines inactivates class I histone deacetylases 1, 2, and 3 and antagonizes their transcriptional repressor function. J Biol Chem 285:17417-24 (2010). WB . Read more (PubMed: 20385560) »
  • Imbriano C  et al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol 25:3737-51 (2005). Read more (PubMed: 15831478) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab47237.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"