Overview

  • Product nameAnti-HDAC3 antibody [EP462Y]
    See all HDAC3 primary antibodies
  • Description
    Rabbit monoclonal [EP462Y] to HDAC3
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of Histone deacetylase 3.

  • Positive control
    • HeLa nuclear lysate Human breast carcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Associated products

Applications

Our Abpromise guarantee covers the use of ab76295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
    • Post-translational
      modifications
      Sumoylated in vitro.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HD3 antibody
      • HDAC 3 antibody
      • HDAC3 antibody
      • HDAC3_HUMAN antibody
      • Histone deacetylase 3 antibody
      • RPD3 2 antibody
      • RPD3 antibody
      • RPD3-2 antibody
      • SMAP45 antibody
      see all

    Anti-HDAC3 antibody [EP462Y] images

    • Anti-HDAC3 antibody [EP462Y] (ab76295) at 1/2000 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
    • ab76295, at a 1/100-1/250 dilution, staining human HDAC3 in breast carcinoma, by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    References for Anti-HDAC3 antibody [EP462Y] (ab76295)

    This product has been referenced in:
    • Choi HK  et al. PINK1 positively regulates HDAC3 to suppress dopaminergic neuronal cell death. Hum Mol Genet 24:1127-41 (2015). Read more (PubMed: 25305081) »
    • Sleiman SF  et al. Histone Deacetylase Inhibitors and Mithramycin A Impact a Similar Neuroprotective Pathway at a Crossroad between Cancer and Neurodegeneration. Pharmaceuticals (Basel) 4:1183-1195 (2011). WB ; Rat . Read more (PubMed: 22582024) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"