Anti-HDAC3 (phospho S424) antibody (ab61056)

Overview

  • Product nameAnti-HDAC3 (phospho S424) antibody
    See all HDAC3 primary antibodies
  • Description
    Rabbit polyclonal to HDAC3 (phospho S424)
  • Specificityab61056 detects endogenous levels of HDAC3 only when phosphorylated at serine 424.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human HDAC3 around the phosphorylation site of serine 424 (KESpDV).

  • Positive control
    • NIH/3T3 cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab61056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ELISA 1/5000.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated in vitro.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

Anti-HDAC3 (phospho S424) antibody images

  • All lanes : Anti-HDAC3 (phospho S424) antibody (ab61056) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract with no phosphopeptide
    Lane 2 : NIH/3T3 cell extract with phosphopeptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa

References for Anti-HDAC3 (phospho S424) antibody (ab61056)

ab61056 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lymphoblastoid cell line)
Loading amount 50 µg
Specification lymphoblastoid cell line
Gel Running Conditions Non-reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Apr 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"