Overview

  • Product nameAnti-HDAC4 antibody
    See all HDAC4 primary antibodies
  • Description
    Rabbit polyclonal to HDAC4
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HDAC4 aa 1-18.
    Sequence:

    MSSQSHPDGLSGRDQPVE

  • Positive control
    • Hela cell lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2

Properties

Applications

Our Abpromise guarantee covers the use of ab16339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 140 kDa.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHO3 antibody
    • BDMR antibody
    • EC 3.5.1.98 antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all

Anti-HDAC4 antibody images

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human skin tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Rabbit Polyclonal Antibody recognizing HDAC4 (ab16339 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • ab16339 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16339 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.



  • Predicted band size : 140 kDa
    ab16339 at 1/1000 detecting HDAC4 from HeLa cell lysate by Western blot

References for Anti-HDAC4 antibody (ab16339)

ab16339 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I wish to apologize for the confusion as I had made this mistake when creating the recall email. The correct immunogen does correspond to aa1-18 of human HDAC4 and not to the peptide stated above. Thank you ver...

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Thank you very much for sharing the results you obtained with ab32073. I am very pleased to hear that it has worked well for you.

It would be very useful for other customers if you could share this information in the form of an Abreview? This...

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Thank you for your reply and understanding. I emailed as quickly as I could after receiving the information so that you wouldn't be running blots unnecessarily.

I have now arranged for the rabbit monoclonal ab32073 to be sent to you. This is ...

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Thank you for getting back to me.

As I think I mentioned to you, I was consulting with the source of this antibody to get confirmation of the immunogen used to raise the antibody.

Unfortunately, they have gone back in their records ...

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Thank you for getting back to me. If you wouldn't mind testing ab16339 again I think that would be worthwhile, just to confirm there was no mix up along the way. If you get the same results again I'd be happy to send ab32073 if you would like to try it...

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Thank you for providing that information. It has made understanding the problems encountered in your experiment much easier to understand.

As we discussed over the phone, it is very unlikely that you have been provided with the wrong vial of ...

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