Overview

  • Product nameAnti-HDAC4 antibody - ChIP Grade
    See all HDAC4 primary antibodies
  • Description
    Rabbit polyclonal to HDAC4 - ChIP Grade
  • SpecificityThe antibody detects ~140 kDa histone deacetylase 4. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 5, 6, 7, and 8.
  • Tested applicationsSuitable for: IHC-FoFr, ICC/IF, WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    This information is considered to be commercially sensitive(Peptide available as ab53331.)

  • General notesSome tissues may not express the HDAC-4 and 5 protein or it is expressed at extremely low amount. HDAC-1,2,3,6,7 are usually much more abundant (could be ~100X more) than HDAC-4 & 5. We would strongly suggest using positive control (HeLa lysate).

Properties

Applications

Our Abpromise guarantee covers the use of ab1437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 24465754
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa.Can be blocked with HDAC4 peptide (ab53331).
ChIP Use 1µl for 105 cells. PubMed: 15990875

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHO3 antibody
    • BDMR antibody
    • EC 3.5.1.98 antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all

Anti-HDAC4 antibody - ChIP Grade images

  • ICC/IF image of ab1437 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1437, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-HDAC4 antibody - ChIP Grade (ab1437) at 1/500 dilution

    Lane 1 : HDAC4 KO whole mouse brain (P3)
    Lane 2 : WT whole mouse brain (P3)

    Lysates/proteins at 30 µg per lane.

    Secondary
    Anti-Rabbit IgG, HRP-conjugated F(ab')2 Fragment at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 119 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-HDAC4 antibody - ChIP Grade (ab1437)

This product has been referenced in:
  • Yang Y  et al. The interaction of the atm genotype with inflammation and oxidative stress. PLoS One 9:e85863 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 24465754) »
  • Sharma A  et al. Calcium-mediated histone modifications regulate alternative splicing in cardiomyocytes. Proc Natl Acad Sci U S A 111:E4920-8 (2014). Read more (PubMed: 25368158) »

See all 9 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - BD permeabilization buffer
Sample Mouse Cell (Mouse thymus tissue, thymocytes)
Specification Mouse thymus tissue, thymocytes
Gating Strategy CD45 positives
Preparation Cell harvesting/tissue preparation method: Digestion with Collagenase D
Sample buffer: PBS 2% FCS
Username

Mr. Sanjay Gawade

Verified customer

Submitted Jan 26 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse brain)
Loading amount 20 µg
Specification Mouse brain
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (whole brain p3)
Loading amount 30 µg
Specification whole brain p3
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 06 2012

Thank you for calling Abcam earlier today. I have recieved a reply from the lab about the region where ab1437 binds. They have told me that the anitbody was generated using a synthetic peptide corresponding to residues surrounding amino acid 10 of huma...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (thymocytes, fibroblasts, hek293, jurkat)
Specification thymocytes, fibroblasts, hek293, jurkat
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step Real-time PCR
Positive control Cells that express HBP1, a protein that has been shown to interact with HDAC4 and primers that amplify the HBP1 binding site
Negative control Same cells but primers that ampify an irrelevant region or cell type that does not express HBP1 (the interacting protein)
Username

Dr. Eleni Ktisstaki

Verified customer

Submitted Oct 25 2006

Thank you for getting back to me and clarifying this issue. Once again I am surprised by your observed lack of expression of HDAC4 in HeLa cells given the literature that reports its expression in these cells. I would like to offer you a replacement...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and you are largely following a protocol that I would recommend. Furthermore according to the literatu...

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Thank you for your enquiry. The originator of this antibody has told me that they sometimes see two weaker bands at 80-100 kDa and another one at 40-60 kDa. They think these could be the cleavage fragments of HDAC4. Please note that some tissues ma...

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Thank you for the information. I have instructed our accounting department to issue you a refund for ab1440 and ab1437. This process does take a little while and if you have any questions regarding the refund, please contact our accounts department at ...

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Thank you for the information regarding ab1440. I searched through our system and I don't have a record of an enquiry from you regarding ab1437. Can you please send me a description of the problem with ab1437 and your protocol? I will then investigate ...

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