Overview

  • Product nameAnti-HDAC4 antibody - ChIP Grade
    See all HDAC4 primary antibodies
  • Description
    Rabbit polyclonal to HDAC4 - ChIP Grade
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, ChIP, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide:

    CISSDPRYWYGKTQHS

    , corresponding to amino acids 194-209 of Human HDAC4

  • Positive control
    • Lung, respiratory epithelium.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300. PubMed: 23469282
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 119 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21526203

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHO3 antibody
    • BDMR antibody
    • EC 3.5.1.98 antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all

Anti-HDAC4 antibody - ChIP Grade images

  • ab79521 at 5µg/ml staining HDAC4 by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded lung, respiratory epithelium sections).

References for Anti-HDAC4 antibody - ChIP Grade (ab79521)

This product has been referenced in:
  • Shen W  et al. Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated Fatty acids. Sci Rep 4:5282 (2014). ChIP ; Mouse . Read more (PubMed: 24923522) »
  • Takase K  et al. Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases. PLoS One 8:e58473 (2013). ICC/IF ; Mouse . Read more (PubMed: 23469282) »

See all 3 Publications for this product

Product Wall

Je suis ravie que vous souhaitiez participer a notre offre et tester ab79521 en IHC-Fr. Le code de réduction correspondant est xx . Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire. Vous devez dans un pre...

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Merci de votre intérêt pour nos anticorps. Comme discuté au téléphone, à ma connaissance, aucun de nos anticorps contre le HDAC4 à été déjà testé sur coupes congélées classiques (IHC-Fr). Si vous souhaitez donc tester ab79521 en IHC-Fr, il me sera poss...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"