- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-HDAC4 (phospho S632) antibody
See all HDAC4 primary antibodies -
DescriptionRabbit polyclonal to HDAC4 (phospho S632)
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Host speciesRabbit
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Specificityab39408 detects endogenous levels of HDAC4 only when phosphorylated at serine 632.
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of serine 632 (A-Q-SP-S-P)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab39408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 119 kDa. | |
| ELISA | 1/20000. |
Target
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FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
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Tissue specificityUbiquitous.
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Involvement in diseaseDefects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
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Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
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DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
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Post-translational
modificationsPhosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4. -
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
- Information by UniProt
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Database links
- Entrez Gene: 9759 Human
- Entrez Gene: 208727 Mouse
- Entrez Gene: 363287 Rat
- Omim: 605314 Human
- SwissProt: P56524 Human
- SwissProt: Q6NZM9 Mouse
- SwissProt: Q99P99 Rat
- Unigene: 20516 Human
see all -
Alternative names
- AHO3 antibody
- BDMR antibody
- EC 3.5.1.98 antibody
see all
Images
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Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408)All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution
Lane 1 : Extract from Jurkat cells (5-30ug of total protein)
Lane 2 : Extract from Jurkat cells treated with Calyculin A (5-30ug of total protein)
Predicted band size: 119 kDa -
Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408)Image from Evankovich J et al, J Biol Chem. 2010 Dec 17;285(51):39888-97. Epub 2010 Oct 11, Fig 4.20 µg of nuclear lysate prepared from mouse liver was diluted in SDS buffer and run on SDS-PAGE gel. Samples were transferred to nitrocellulose overnight, and blocked with 5% BSA for 8 hours. They were then incubated overnight with primary antibodies, which included an anti-HDAC1, 1/1000 and ab39408, 1/1000. After washing, membranes were incubated with horseradish peroxidase-coupled rabbit or mouse secondary antibodies (1/5000 to 1/10,000) in 5% milk for 1 hour and developed with a Super Signal West Pico chemiluminescent kit. -
Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408)Image courtesy of an anonymous Abreview.All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution
Lane 1 : Lysate from murine heart tissue 1
Lane 2 : Lysate from murine heart tissue 2
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRp conjugated goat anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 119 kDa
Observed band size: 130 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 minute
Protocols
References
This product has been referenced in:
- Amara S et al. Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation. PLoS One 12:e0180097 (2017). Read more (PubMed: 28658303) »
- Popescu I et al. Elevated local [Ca2+] and CaMKII promote spontaneous Ca2+ release in ankyrin-B-deficient hearts. Cardiovasc Res 111:287-94 (2016). Read more (PubMed: 27131508) »
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