Anti-HDAC4 (phospho S632) antibody (ab39408)

Overview

• Product nameAnti-HDAC4 (phospho S632) antibody
  See all HDAC4 primary antibodies
• Description
  Rabbit polyclonal to HDAC4 (phospho S632)
• Specificityab39408 detects endogenous levels of HDAC4 only when phosphorylated at serine 632.
• Tested applicationsSuitable for: WB, ELISAmore details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of serine 632 (A-Q-S^P-S-P)

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg⁺⁺ and Ca⁺⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• ClonalityPolyclonal
• IsotypeIgG
• Research areas
  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • HDACs

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HDACs
  • Class II / Hda1 Class

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
• Tissue specificityUbiquitous.
• Involvement in diseaseDefects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
• Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
• DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
• Post-translational
  modificationsPhosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
  Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
• Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.

• Target information above from: UniProt accession P56524 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 9759 Human
  • Entrez Gene: 208727 Mouse
  • Entrez Gene: 363287 Rat
  • Omim: 605314 Human
  • SwissProt: P56524 Human
  • SwissProt: Q6NZM9 Mouse
  • SwissProt: Q99P99 Rat
  • Unigene: 20516 Human

  • Unigene: 318567 Mouse
  • Unigene: 23483 Rat

  see all
• Alternative names
  • AHO3 antibody
  • BDMR antibody
  • EC 3.5.1.98 antibody

  • HA6116 antibody
  • HD 4 antibody
  • HD4 antibody
  • HDAC 4 antibody
  • HDAC A antibody
  • HDAC4 antibody
  • HDAC4_HUMAN antibody
  • HDACA antibody
  • Histone deacetylase 4 antibody
  • Histone Deacetylase A antibody
  • KIAA0288 antibody

  see all