Anti-HDAC4 (phospho S632) antibody (ab39408)

Overview

  • Product nameAnti-HDAC4 (phospho S632) antibody
    See all HDAC4 primary antibodies
  • Description
    Rabbit polyclonal to HDAC4 (phospho S632)
  • Specificityab39408 detects endogenous levels of HDAC4 only when phosphorylated at serine 632.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of serine 632 (A-Q-SP-S-P)

Properties

Applications

Our Abpromise guarantee covers the use of ab39408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 119 kDa.
ELISA 1/20000.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHO3 antibody
    • BDMR antibody
    • EC 3.5.1.98 antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all

Anti-HDAC4 (phospho S632) antibody images

  • All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution

    Lane 1 : Extract from Jurkat cells (5-30ug of total protein)
    Lane 2 : Extract from Jurkat cells treated with Calyculin A (5-30ug of total protein)


    Predicted band size : 119 kDa


  • Predicted band size : 119 kDa

    Image from Evankovich J et al, J Biol Chem. 2010 Dec 17;285(51):39888-97. Epub 2010 Oct 11, Fig 4.

  • All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution

    Lane 1 : Lysate from murine heart tissue 1
    Lane 2 : Lysate from murine heart tissue 2

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRp conjugated goat anti-rabbit polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 119 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

References for Anti-HDAC4 (phospho S632) antibody (ab39408)

This product has been referenced in:
  • Li H  et al. Calmodulin kinase II is required for angiotensin II-mediated vascular smooth muscle hypertrophy. Am J Physiol Heart Circ Physiol 298:H688-98 (2010). Read more (PubMed: 20023119) »
  • Evankovich J  et al. High Mobility Group Box 1 Release from Hepatocytes during Ischemia and Reperfusion Injury Is Mediated by Decreased Histone Deacetylase Activity. J Biol Chem 285:39888-97 (2010). WB ; Mouse . Read more (PubMed: 20937823) »

See all 3 Publications for this product

Product Wall

Thank you very much for your reply.
It looks like there may be a band around the expected molecular weight that is more visible in the even lanes compared to the odd lanes (except for lane 9, it looks like the band is also in this lane). However i...

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Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (5)
Sample Rat Cell lysate - whole cell (Hippocampal primary Neurons)
Specification Hippocampal primary Neurons
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

Submitted Nov 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse brain)
Loading amount 20 µg
Specification Mouse brain
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 01 2013

Thank you for your reply. It is definitely possible that you may notice a shift in the molecular weight. We have not tested this extensively with our general HDAC5 antibodies, but it could explain the extra bands in lane three of the WB image for ab119...

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Thank you very much for your reply and for sending this additional information.
I do have a couple more questions. What gene is being transduced in these L6 cells? Were the Western blot results similar in the control cells?
We have not yet te...

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Thank you for contacting us and letting us know about the trouble with ab53693.
I do have a few questions so that I can better understand the situation:
1) What kind of samples were used? What species and cell/tissue type? Were they treated t...

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Thank you for contacting us. We have only tested this antibody in HeLa cells for IF. The results were not very convincing, but if your customer is still interested in testing it with a different cell line, I can offer him/her a testing discount to perf...

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Thank you for your phone call the other day and for your patience while I have been in touch with the lab about ab39408. For the positive control Jurkat cells, the following protocol was used: A. Jurkat cells were plated on flask or dishes conta...

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Thank you for your enquiry. I have contacted the originator of the product but unfortunately, they do not know of a good positive control for this product. However, the product has been tested in Jurkat cells treated with Calyculin A and they woul...

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