The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
Use at an assay dependent concentration.
1/100. PubMed: 23469282
Use at an assay dependent concentration. PubMed: 21526203
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Post-translational modificationsPhosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using ab55403 (1/50 dilution) in the presence and absence of the immunizing peptide.
Western blot - HDAC5 antibody (ab55403)
All lanes : Anti-HDAC5 antibody (ab55403) at 1/500 dilution
Lane 1 : HepG2 cell extract, without immunizing peptide Lane 2 : HepG2 cell extract, with immunizing peptide
Predicted band size : 122 kDa Observed band size : 122 kDa
References for Anti-HDAC5 antibody (ab55403)
This product has been referenced in:
Nichols CB et al. ß-adrenergic signaling inhibits Gq-dependent protein kinase D activation by preventing protein kinase D translocation. Circ Res114:1398-409 (2014).
Read more (PubMed: 24643961) »
Takase K et al. Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases. PLoS One8:e58473 (2013).
Read more (PubMed: 23469282) »