Overview

  • Product name
    Anti-HDAC5 antibody [HDAC5-35]
    See all HDAC5 primary antibodies
  • Description
    Mouse monoclonal [HDAC5-35] to HDAC5
  • Tested applications
    Suitable for: ICC/IF, ELISA, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human HDAC5 aa 4-19 conjugated to keyhole limpet haemocyanin. synthetic peptide has a C-terminal added lysine.
    Sequence:

    PNESDGMSGREPSLEI


    Database link: Q9UQL6

  • Positive control
    • WB: Total cell extracts of NIH3T3 fibroblast cells.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab55403 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab50001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 122 kDa.

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Domain
    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen NY CO 9 antibody
    • Antigen NY-CO-9 antibody
    • HD5 antibody
    • HDAC 5 antibody
    • HDAC5 antibody
    • HDAC5_HUMAN antibody
    • Histone deacetylase 5 antibody
    • NY CO 9 antibody
    see all

Images

  • Lane 1 : Anti-HDAC5 antibody [HDAC5-35] (ab50001) at 2 µg/ml
    Lane 2 : no primary antibody

    Lane 1 : 3T3 cell extract (mouse fibroblasts)
    Lane 2 : 3T3 cell extract (mouse fibroblasts)

    Secondary
    Goat Anti-Mouse, Peroxidase conjugate

    Predicted band size : 122 kDa
    Observed band size : ~122 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Mitsi V  et al. RGS9-2--controlled adaptations in the striatum determine the onset of action and efficacy of antidepressants in neuropathic pain states. Proc Natl Acad Sci U S A 112:E5088-97 (2015). WB ; Mouse . Read more (PubMed: 26305935) »

See 1 Publication for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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