Anti-HDAC6 antibody (ab117516)


  • Product nameAnti-HDAC6 antibody
    See all HDAC6 primary antibodies
  • Description
    Rabbit polyclonal to HDAC6
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids in Human HDAC6 (NP_006035.2).

  • Positive control
    • Human intestine (ganglion cells) and Human testis tissues; HepG2 cells.



Our Abpromise guarantee covers the use of ab117516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 131 kDa.
ELISA 1/1000.
IHC-P 1/200.
ICC/IF 1/100 - 1/500.


  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational
    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localizationNucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all

Anti-HDAC6 antibody images

  • ab117516, at 1:200 dilution, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human intestine tissue (ganglion cells) by Immunohistochemistry.

  • ab117516, at 1:200 dilution, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry.

  • All lanes : Anti-HDAC6 antibody (ab117516) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with immunizing peptide

    Predicted band size : 131 kDa
  • ab117516, at 1/100, staining HDAC6 in HepG2 cells by Immunofluorescence (Left image).
    The image on the right is treated with the synthesized peptide.

References for Anti-HDAC6 antibody (ab117516)

ab117516 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - nuclear (LNCaP - Prostate Cancer Cells)
Gel Running Conditions Reduced Denaturing (4-15% SDS PAGE)
Loading amount 35 µg
Specification LNCaP - Prostate Cancer Cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted May 09 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (LNCaP - prostate cancer cells)
Permeabilization Yes - 0.2% Triton X-100 in PBS
Specification LNCaP - prostate cancer cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Apr 07 2017

Thank you for your enquiry. I am sorry to confirm that as far as we are aware,  ab117516  Anti-HDAC6 antibody has not been tested and is therefore not guaranteed in mouse. I would be very pleased to hear to provide you with a discount code for the cust...

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