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Overview
Product name
Anti-HDAC6 antibody
See all HDAC6 products (13) ...
Description
Rabbit polyclonal to HDAC6
Tested applications
ICC/IF, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: KNIAHQNKFGEDMPH conjugated to KLH, corresponding to amino acids 1199-1213 of Human HDAC6.
KNIAHQNKFG EDMPH
Positive control
293T cells expressing recombinant mouse HDAC6.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (Use at a dilution of 1/50 for 1 hr.)
WB: 1/1000Detects a band of approximately 134 kDa.
IP: Use at an assay dependent dilution.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
Post-translational
modifications
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
Target information above from: UniProt accessionQ9UBN7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ16239 antibody
- HD 6 antibody
- HD6 antibody
see all
Database links
- Entrez Gene: 10013 Human
- Entrez Gene: 15185 Mouse
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9Z2V5 Mouse
- Unigene: 6764 Human
- Unigene: 29854 Mouse
Anti-HDAC6 antibody images:
Western blot - HDAC6 antibody (ab12173)
All lanes : Anti-HDAC6 antibody (ab12173) at 1/500 dilution
Lane 1 : Whole cell lysate prepared from Human HepG2 cell line.
Lane 2 : Whole cell lysate prepared from Human HepG2 cell line.
Lane 3 : Whole cell lysate prepared from Human HepG2 cell line.
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated goat anti-rabbit IgG at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an Abreview submitted by Cheng Song
Immunocytochemistry/ Immunofluorescence - HDAC6 antibody (ab12173)
ab12173 staining mouse NIH3T3 cells by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C . The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C . A Alexa Fluor® 488 goat polyclonal to rabbit IgG was used as the secondary, diluted 1/200.
This image is courtesy of an anonymous Abreview
References for Anti-HDAC6 antibody (ab12173)
This product has been referenced in:
- Ouyang Het al. Protein Aggregates Are Recruited to Aggresome by Histone Deacetylase 6 via Unanchored Ubiquitin C Termini. J Biol Chem 287:2317-27 (2012).Read more (PubMed: 22069321) »
- Alamdari Net al. Sepsis and glucocorticoids upregulate p300 and downregulate HDAC6 expression and activity in skeletal muscle. Am J Physiol Regul Integr Comp Physiol 299:R509-20 (2010). WB.Read more (PubMed: 20538901) »
See all 2 publications for this product
Publishing research using ab12173? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"