Overview

  • Product name
  • Description
    Rabbit polyclonal to HDAC6
  • Specificity
    The antibody detects ~134 kDa histone deacetylase 6. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Mouse) - N terminal.

  • Positive control
    • WB: Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab1440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa).Can be blocked with HDAC6 peptide (ab41708). Block with 5% milk in TBST and incubate the antibody in blocking buffer.

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational
    modifications
    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localization
    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all

Images

  • Anti-HDAC6 antibody (ab1440) + Jurkat cell lysate

    Predicted band size : 131 kDa
    Observed band size : 131 kDa

References

This product has been referenced in:
  • Zan J  et al. Rabies Virus Infection Induces Microtubule Depolymerization to Facilitate Viral RNA Synthesis by Upregulating HDAC6. Front Cell Infect Microbiol 7:146 (2017). WB ; Mouse . Read more (PubMed: 28491824) »
  • Stoney PN  et al. A seasonal switch in histone deacetylase gene expression in the hypothalamus and their capacity to modulate nuclear signaling pathways. Brain Behav Immun 61:340-352 (2017). Read more (PubMed: 27993690) »

See all 12 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
Sample
Mouse Cell (Neural cell)
Specification
Neural cell
Permeabilization
Yes - 0.3% TritonX-100 in PBS for 30 minutes
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 20 2014

Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Loading amount
20 µg
Specification
Primary Neurons
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 15 2013

Thank you for your reply.

I looked up the order associated with ab1440, and it look as though you received that product for free as part of our 4for3 promotion a month ago. Since you received it for free, there is no cost to refund, but I am ...

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Thank you for providing that image.

Based on the information you have provided, I do beleive that there a re protocol varations that I can suggest that will help resolve this situation, therefore I would be happy to replace or refund the cost...

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Thank you for contacting Abcam.

I am sorry that you are having some issues with ab1440. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot resolve the issue you...

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Thank you for your enquiry. I have been in touch with our lab team and they have informed me that for the application of this antibody by IHC using paraffin embedded sections antigen retrieval should be performed by heating sections in a microwave ...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1440. This antibody has been tested with HeLa cell lysate (20 ug) and a ~134 kDa band was detected. The antibody should with mouse samples as well. Can you tel...

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The immunogen used was a synthetic peptide mapping to Mouse HDAC-6 N-terminus. If you have any additional questions, please contact us again.

Thank you for the information. I have instructed our accounting department to issue you a refund for ab1440 and ab1437. This process does take a little while and if you have any questions regarding the refund, please contact our accounts department at ...

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Thank you for the information regarding ab1440. I searched through our system and I don't have a record of an enquiry from you regarding ab1437. Can you please send me a description of the problem with ab1437 and your protocol? I will then investigate ...

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