Anti-HDAC6 antibody (ab233520)
Key features and details
- Rabbit polyclonal to HDAC6
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-HDAC6 antibody
See all HDAC6 primary antibodies -
Description
Rabbit polyclonal to HDAC6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-tag) corresponding to Human HDAC6 aa 150-350. N-terminal tag. (Expressed in E.coli).
Database link: Q9UBN7-1 -
Positive control
- WB: Pig kidney lysate; Recombinant human HDAC6 protein. IHC-P: Human kidney, liver and liver cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
Post-translational
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
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Database links
- Entrez Gene: 10013 Human
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- Unigene: 6764 Human
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Alternative names
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for HDAC6 using ab233520 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver cancer tissue stained for HDAC6 using ab233520 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for HDAC6 using ab233520 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-HDAC6 antibody (ab233520) at 2 µg/ml + Pig kidney lysate
Secondary
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution -
Anti-HDAC6 antibody (ab233520) at 2 µg/ml + Recombinant human HDAC6 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233520 has not yet been referenced specifically in any publications.