HDAC6 overexpression 293T lysate (whole cell) (ab94173)

Overview

  • Product nameHDAC6 overexpression 293T lysate (whole cell)
  • Description
    HDAC6 overexpression lysate
  • General notesab94173 is a 293T cell transfected lysate in which Human HDAC6 has been transiently over-expressed using a pCMV-HDAC6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Similarity: Belongs to the histone deacetylase family. HD type 2 subfamily. Contains 1 UBP-type zinc finger. PTM: Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro.

Applications

Our Abpromise guarantee covers the use of ab94173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

HDAC6 overexpression 293T lysate (whole cell) images

  • ab94173 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-HDAC6 antibody (ab56926) at 1/500 dilution

    Lane 1 : HDAC6 overexpression 293T lysate (whole cell) (ab94173)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for HDAC6 overexpression 293T lysate (whole cell) (ab94173)

ab94173 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"