Overview

  • Product nameAnti-HDAC6 (phospho S22) antibody
    See all HDAC6 primary antibodies
  • Description
    Rabbit polyclonal to HDAC6 (phospho S22)
  • Specificityab61058 detects endogenous levels of HDAC6 only when phosphorylated at serine 22. (Human: Ser22; Mouse: Ser21).
  • Tested applicationsSuitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human HDAC6 around the phosphorylation site of serine 22 (PQSpPP).

  • Positive control
    • Human testis tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab61058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/5000.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational
    modifications
    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localizationNucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all

Anti-HDAC6 (phospho S22) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human testis tissue using HDAC6 (phospho S22) antibody (ab61058) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of phosphopeptide.

References for Anti-HDAC6 (phospho S22) antibody (ab61058)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (preadipocyte)
Loading amount 4 µg
Specification preadipocyte
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Hongzhong Wang

Verified customer

Submitted Feb 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"