ab61058 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Belongs to the histone deacetylase family. HD type 2 subfamily. Contains 1 UBP-type zinc finger.
Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro.
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
Immunohistochemistry analysis of paraffin-embedded human testis tissue using HDAC6 (phospho S22) antibody (ab61058) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of phosphopeptide.