• Product nameAnti-HDAC7 antibody
    See all HDAC7 primary antibodies
  • Description
    Rabbit polyclonal to HDAC7
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    with N-terminal added lysine, conjugated to KLH, corresponding to amino acids 137-155 of Mouse HDAC7. The corresponding sequence is identical in rat and differs by three amino acids in human.

  • Positive control
    • Mouse NIH-3T3 cells, whole extracts of rat PC12 cells (Western blotting)
  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.



Our Abpromise guarantee covers the use of ab12175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 105 kDa. Additional weak bands may be detected in various extract preparations. A minimum working antibody dilution of 1/2000 is recommended using whole extracts of mouse NIH-3T3 cells. A minimum working antibody dilution of 1/500 is recommended using whole extracts of rat PC12 cells.
IP Use at an assay dependent dilution. 1.5-1.8 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T human embryonic kidney cells expressing recombinant human HDAC7.


  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
  • Cellular localizationNucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

References for Anti-HDAC7 antibody (ab12175)

This product has been referenced in:
  • Jensen ED  et al. Bone morphogenic protein 2 activates protein kinase D to regulate histone deacetylase 7 localization and repression of Runx2. J Biol Chem 284:2225-34 (2009). IP ; Mouse . Read more (PubMed: 19029091) »

See 1 Publication for this product

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