Overview

  • Product nameAnti-HDAC7 antibody
    See all HDAC7 primary antibodies
  • Description
    Rabbit polyclonal to HDAC7
  • SpecificityThe antibody detects ~105 kDa histone deacetylase 7. It does not cross-react with other HDAC proteins.
  • Tested applicationsSuitable for: IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    GILAEDRPSERLVE

    (Mouse)

Properties

Applications

Our Abpromise guarantee covers the use of ab1441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 30 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 102.9 kDa.
IP Use a concentration of 20 - 40 µg/ml.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
  • Cellular localizationNucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

References for Anti-HDAC7 antibody (ab1441)

This product has been referenced in:
  • Chang S  et al. Histone deacetylase 7 maintains vascular integrity by repressing matrix metalloproteinase 10. Cell 126:321-34 (2006). ChIP ; Human . Read more (PubMed: 16873063) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - BD permeabilization buffer
Sample Mouse Cell (Mouse thymus tissue)
Specification Mouse thymus tissue
Gating Strategy CD45 positives
Preparation Cell harvesting/tissue preparation method: Digestion with Collagenase D
Sample buffer: PBS 2% FCS
Username

Mr. Sanjay Gawade

Verified customer

Submitted Jan 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"