Overview

  • Product nameAnti-HDAC7 antibody
    See all HDAC7 primary antibodies
  • Description
    Rabbit polyclonal to HDAC7
  • Specificityab53101 detects endogenous levels of total HDAC7 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from human HDAC7.

  • Positive control
    • IHC-P: Human lung carcinoma tissue WB: HUVEC cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab53101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
  • Cellular localizationNucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

Anti-HDAC7 antibody images

  • ab53101 staining HDAC7 in human rhabdomyosarcoma (soft tissue sarcoma) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 10 minutes at 25°C; antigen retrieval was enzymatic using proteinase K buffer for 5 minutes at 37oC. Samples were incubated with primary antibody (1/75) for 20 minutes at 25°C. An undiluted HRP-conjugated goat anti-mouse/rabbit IgG polyclonal was used as the secondary antibody.

  • ab53096, at a 1/50 dilution, staining HDAC7 in paraffin embedded human lung carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.ab53096, at a 1/50 dilution, staining HDAC7 in paraffin embedded human lung carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
  • All lanes : Anti-HDAC7 antibody (ab53101) at 1/300 dilution

    Lane 1 : HUVEC cell extract
    Lane 2 : HUVEC cell extract with peptide


    Predicted band size : 103 kDa
    Observed band size : 103 kDa

References for Anti-HDAC7 antibody (ab53101)

ab53101 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (rhabdomyosarcoma)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K, 5 min, 37C
Specification rhabdomyosarcoma
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
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Verified customer

Submitted Jan 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mouse liver)
Loading amount 20 µg
Specification mouse liver
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

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Submitted Mar 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (aortic smooth muscle)
Loading amount 5 µg
Specification aortic smooth muscle
Gel Running Conditions Reduced Denaturing (7.5% gel)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted Feb 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"