The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/300 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
Use at an assay dependent concentration.
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
Belongs to the histone deacetylase family. HD type 2 subfamily.
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC7 antibody (ab53101)This image is courtesy of an anonymous abreview.
ab53101 staining HDAC7 in human rhabdomyosarcoma (soft tissue sarcoma) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 10 minutes at 25°C; antigen retrieval was enzymatic using proteinase K buffer for 5 minutes at 37oC. Samples were incubated with primary antibody (1/75) for 20 minutes at 25°C. An undiluted HRP-conjugated goat anti-mouse/rabbit IgG polyclonal was used as the secondary antibody.
ab53096, at a 1/50 dilution, staining HDAC7 in paraffin embedded human lung carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.ab53096, at a 1/50 dilution, staining HDAC7 in paraffin embedded human lung carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
Western blot - HDAC7 antibody (ab53101)
All lanes : Anti-HDAC7 antibody (ab53101) at 1/300 dilution
Lane 1 : HUVEC cell extract Lane 2 : HUVEC cell extract with peptide
Predicted band size : 103 kDa Observed band size : 103 kDa