Overview

  • Product name
    Anti-HDAC7 antibody [EPR10922]
    See all HDAC7 primary antibodies
  • Description
    Rabbit monoclonal [EPR10922] to HDAC7
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human HDAC7.

  • Positive control
    • HeLa, A549, Human fetal brain and K562 cell lysate. K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab166911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 103 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
    • Sequence similarities
      Belongs to the histone deacetylase family. HD type 2 subfamily.
    • Domain
      The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
    • Post-translational
      modifications
      May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    • Cellular localization
      Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZP586J0917 antibody
      • FLJ99588 antibody
      • HD 7a antibody
      • HD7 antibody
      • HD7a antibody
      • HDAC 7 antibody
      • HDAC 7A antibody
      • Hdac7 antibody
      • HDAC7_HUMAN antibody
      • HDAC7A antibody
      • Histone deacetylase 7 antibody
      • Histone deacetylase 7A antibody
      • OTTHUMP00000202813 antibody
      • OTTHUMP00000202814 antibody
      see all

    Images

    • All lanes : Anti-HDAC7 antibody [EPR10922] (ab166911) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : A549 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 103 kDa
    • Flow cytometric analysis of permeabilized K562 cells labeling HDAC7 with ab166911 at 1/100 dilution (red) or a rabbit IgG control (negative) (green).

    References

    This product has been referenced in:
    • Mondello P  et al. Panobinostat acts synergistically with ibrutinib in diffuse large B cell lymphoma cells with MyD88 L265 mutations. JCI Insight 2:e90196 (2017). WB ; Human . Read more (PubMed: 28352655) »
    • Galán M  et al. Induction of histone deacetylases (HDACs) in human abdominal aortic aneurysm: therapeutic potential of HDAC inhibitors. Dis Model Mech 9:541-52 (2016). WB . Read more (PubMed: 26989193) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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