Anti-HDAC7 antibody [HDAC7-97] - ChIP Grade (ab50212)


  • Product nameAnti-HDAC7 antibody [HDAC7-97] - ChIP Grade
    See all HDAC7 primary antibodies
  • Description
    Mouse monoclonal [HDAC7-97] to HDAC7 - ChIP Grade
  • Tested applicationsSuitable for: ELISA, IP, ICC, WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 137-155 of Mouse HDAC7

  • Positive control
    • WB: human embryonal kidney 293T cells expressing recombinant human HDAC7.



Our Abpromise guarantee covers the use of ab50212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 103 kDa.
ChIP Use at an assay dependent dilution. PubMed: 20452320


  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
  • Cellular localizationNucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

Anti-HDAC7 antibody [HDAC7-97] - ChIP Grade images

  • Anti-HDAC7 antibody [HDAC7-97] - ChIP Grade (ab50212) at 0.25 µg/ml + embryonal kidney 293T cells

    Predicted band size : 103 kDa

References for Anti-HDAC7 antibody [HDAC7-97] - ChIP Grade (ab50212)

This product has been referenced in:
  • Shen W  et al. Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated Fatty acids. Sci Rep 4:5282 (2014). ChIP ; Mouse . Read more (PubMed: 24923522) »
  • Muralidhar SA  et al. Histone deacetylase 9 activates gamma-globin gene expression in primary erythroid cells. J Biol Chem 286:2343-53 (2011). WB ; Human . Read more (PubMed: 21078662) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T and LNCaP)
Gel Running Conditions Reduced Denaturing (4-15% TGX BioRad)
Loading amount 200000 cells
Specification HEK293T and LNCaP
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

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Submitted Feb 01 2016