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Abcam’s HDAC8 Activity Assay Kit (Fluorometric) (ab156069) detects HDAC activity in lysates.
Primarily, the HDAC8 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of HDAC inhibitors using recombinant HDAC8. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for HDAC8 activity with the HDAC8 Activity Assay Kit (Fluorometric) after immunoprecipitation with an appropriate HDAC8 specific antibody.
Applications for this kit include:
1. Monitoring the purification of HDACs including HDAC1, 2, 3 and 8 (class I).
2. Screening inhibitors or activators of HDAC8.
3. Detecting the effects of pharmacological agents on HDAC8.
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.
|Developer||1 x 500µl|
|Fluoro-Deacetylated Peptide (0.2 mM)||1 x 100µl|
|Fluoro-Substrate Peptide (0.2 mM)||1 x 500µl|
|HDAC Assay Buffer||2 x 1ml|
|Recombinant HDAC8||1 x 500µl|
|Stop Solution||2 x 1ml|
|Trichostatin A (10µM)||1 x 500µl|
Our Abpromise guarantee covers the use of ab156069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab156069 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"