Anti-HDAC8 (phospho S39) antibody (ab38514)

Overview

  • Product name
    Anti-HDAC8 (phospho S39) antibody
    See all HDAC8 primary antibodies
  • Description
    Rabbit polyclonal to HDAC8 (phospho S39)
  • Specificity
    This antibody is specific for HDAC8 only when phosphorylated at serine 39.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human HDAC8 around the phosphorylation site of serine 39 (RASpMV).

  • Positive control
    • Human lung carcinoma tissue slides.

Properties

Applications

Our Abpromise guarantee covers the use of ab38514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
  • Application notes
    ELISA: 1/20,000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 42 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
    • Tissue specificity
      Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
    • Sequence similarities
      Belongs to the histone deacetylase family. HD type 1 subfamily.
    • Post-translational
      modifications
      Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
    • Cellular localization
      Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDA07 antibody
      • CDLS5 antibody
      • HD 8 antibody
      • HD8 antibody
      • HDAC 8 antibody
      • Hdac8 antibody
      • HDAC8_HUMAN antibody
      • HDACL 1 antibody
      • HDACL1 antibody
      • Histone deacetylase 8 antibody
      • Histone deacetylase like 1 antibody
      • MRXS6 antibody
      • RPD 3 antibody
      • RPD3 antibody
      • WTS antibody
      see all

    Images

    • ab38514 at a 1:50 dilution staining HDAC8 in Human lung carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.

      Left image : untreated.

      Right image : treated with phosphopeptide at 1µg/ml.

    References

    ab38514 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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