Our Abpromise guarantee covers the use of ab18970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 110-140 kDa. A ~75 kDa and a ~60 kDa unknown bands can also be detected in some tissue lysates.|
|IP||Use at an assay dependent dilution.|
ab18970 staining human breast cancer cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% BSA for 1 hour and 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 3 hours at 25°C. A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.