Overview

  • Product nameAnti-HDAC9 antibody
    See all HDAC9 primary antibodies
  • Description
    Rabbit polyclonal to HDAC9
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HDAC9 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Formalin-fixed, paraffin-embedded human breast carcinoma tissue and transfected/ non-transfected Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 111 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
    Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
  • Tissue specificityBroadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
  • Involvement in diseaseNote=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • Post-translational
    modifications
    Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Sumoylated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HD 7 antibody
    • HD 7B antibody
    • HD 9 antibody
    • HD7 antibody
    • HD7B antibody
    • HD9 antibody
    • HDAC 7 antibody
    • HDAC 7B antibody
    • HDAC 9 antibody
    • HDAC 9B antibody
    • HDAC 9FL antibody
    • HDAC antibody
    • HDAC7 antibody
    • HDAC7B antibody
    • HDAC9 antibody
    • HDAC9_HUMAN antibody
    • HDAC9B antibody
    • HDAC9FL antibody
    • HDRP antibody
    • Histone deacetylase 4/5 related protein antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7B antibody
    • Histone deacetylase 9 antibody
    • Histone deacetylase 9A antibody
    • Histone deacetylase related protein antibody
    • Histone deacetylase-related protein antibody
    • KIAA0744 antibody
    • MEF2 interacting transcription repressor MITR antibody
    • MEF2 interacting transcription repressor protein antibody
    • MEF2-interacting transcription repressor MITR antibody
    • MITR antibody
    see all

Anti-HDAC9 antibody images

  • All lanes : Anti-HDAC9 antibody (ab70954) at 1/100 dilution

    Lane 1 : Non-transfected Hela cells
    Lane 2 : HDAC9 transfected Hela cells


    Predicted band size : 111 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue reacted with ab70954 at a 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.

References for Anti-HDAC9 antibody (ab70954)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (small intestine)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20min
Permeabilization No
Specification small intestine
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
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Submitted Jan 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"