Overview

  • Product nameAnti-HDAC9 antibody - ChIP Grade
    See all HDAC9 primary antibodies
  • Description
    Rabbit polyclonal to HDAC9 - ChIP Grade
  • SpecificityThis antibody recognises Histone deacetylase 9, isoform CRA b/isoform 3, also known as HDRP.
  • Tested applicationsSuitable for: WB, IHC-P, ChIP, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Chicken, Guinea pig, Dog
  • Immunogen

    A region within synthetic peptide: QVGAVKVKEE PVDSDEDAQI QEMESGEQAA FMQQVIGKDL APGFVIKVII, corresponding to C terminal amino acids 541-590 of Human HDAC9

  • Positive control
    • A172 cell lysate or human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 20596014
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 25011550

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
    Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
  • Tissue specificityBroadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
  • Involvement in diseaseNote=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • Post-translational
    modifications
    Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Sumoylated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HD 7 antibody
    • HD 7B antibody
    • HD 9 antibody
    • HD7 antibody
    • HD7B antibody
    • HD9 antibody
    • HDAC 7 antibody
    • HDAC 7B antibody
    • HDAC 9 antibody
    • HDAC 9B antibody
    • HDAC 9FL antibody
    • HDAC antibody
    • HDAC7 antibody
    • HDAC7B antibody
    • HDAC9 antibody
    • HDAC9_HUMAN antibody
    • HDAC9B antibody
    • HDAC9FL antibody
    • HDRP antibody
    • Histone deacetylase 4/5 related protein antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7B antibody
    • Histone deacetylase 9 antibody
    • Histone deacetylase 9A antibody
    • Histone deacetylase related protein antibody
    • Histone deacetylase-related protein antibody
    • KIAA0744 antibody
    • MEF2 interacting transcription repressor MITR antibody
    • MEF2 interacting transcription repressor protein antibody
    • MEF2-interacting transcription repressor MITR antibody
    • MITR antibody
    see all

Anti-HDAC9 antibody - ChIP Grade images

  • ChIP analysis using ab59718 (0.5µg/mL) binding HDAC9 in human HCT116 colon cancer cells. Without (- peptide) and with (+ peptide) blocking peptide. Protein binding was detected using real-time PCR.

  • Anti-HDAC9 antibody - ChIP Grade (ab59718) at 1.25 µg/ml + A172 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG used at 1/50000

    Predicted band size : 66 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling HDAC9 with ab59718 at 4µg/ml. Epithelial cells of the renal tubule were positively labelled (indicated with arrows).

References for Anti-HDAC9 antibody - ChIP Grade (ab59718)

This product has been referenced in:
  • Shen W  et al. Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated Fatty acids. Sci Rep 4:5282 (2014). ChIP ; Mouse . Read more (PubMed: 24923522) »
  • Tiwari S  et al. Histone deacetylase expression patterns in developing murine optic nerve. BMC Dev Biol 14:30 (2014). WB, IHC-Fr ; Mouse . Read more (PubMed: 25011550) »

See all 6 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - BD permeabilization buffer
Sample Mouse Cell (Mouse thymus tissue, thymocytes)
Specification Mouse thymus tissue, thymocytes
Gating Strategy CD45 positives
Preparation Cell harvesting/tissue preparation method: Digestion with Collagenase D
Sample buffer: PBS 2% FCS
Username

Mr. Sanjay Gawade

Verified customer

Submitted Jan 26 2015

Thank you for contacting us. I have checked: The immunogen peptide should be available for sale. I am waiting to hear back from the lab what the catalog number and the price will be. Thank you for your patience. The immunogen is a region within synthet...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Cardiomyocytes)
Loading amount 10 µg
Specification Mouse Cardiomyocytes
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Prof. Paul Townsend

Verified customer

Submitted Oct 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"