Validated using a knockout cell line
Recombinant
RabMAb

Anti-HDAC9 antibody [EPR5223] (ab109446)

Overview

  • Product name
    Anti-HDAC9 antibody [EPR5223]
    See all HDAC9 primary antibodies
  • Description
    Rabbit monoclonal [EPR5223] to HDAC9
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of Human HDAC9.

  • Positive control
    • K562, HepG2, and Raji cell lysates; Human colon tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-HDAC9 antibody (Alexa Fluor® 488) [EPR5223] (ab206713)

    Anti-HDAC9 antibody (Alexa Fluor® 647) [EPR5223] (ab207050)

Properties

Applications

Our Abpromise guarantee covers the use of ab109446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 111 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
      Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
    • Tissue specificity
      Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
    • Involvement in disease
      Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
    • Sequence similarities
      Belongs to the histone deacetylase family. HD type 2 subfamily.
    • Post-translational
      modifications
      Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
      Sumoylated.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HD 7 antibody
      • HD 7B antibody
      • HD 9 antibody
      • HD7 antibody
      • HD7B antibody
      • HD9 antibody
      • HDAC 7 antibody
      • HDAC 7B antibody
      • HDAC 9 antibody
      • HDAC 9B antibody
      • HDAC 9FL antibody
      • HDAC antibody
      • HDAC7 antibody
      • HDAC7B antibody
      • HDAC9 antibody
      • HDAC9_HUMAN antibody
      • HDAC9B antibody
      • HDAC9FL antibody
      • HDRP antibody
      • Histone deacetylase 4/5 related protein antibody
      • Histone deacetylase 7 antibody
      • Histone deacetylase 7B antibody
      • Histone deacetylase 9 antibody
      • Histone deacetylase 9A antibody
      • Histone deacetylase related protein antibody
      • Histone deacetylase-related protein antibody
      • KIAA0744 antibody
      • MEF2 interacting transcription repressor MITR antibody
      • MEF2 interacting transcription repressor protein antibody
      • MEF2-interacting transcription repressor MITR antibody
      • MITR antibody
      see all

    Anti-HDAC9 antibody [EPR5223] images



    • Predicted band size : 111 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: HDAC9 (KO) knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)
      Lane 4: Raji whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109446 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109446 was shown to recognize HDAC9 (KO) when HDAC9 (KO) knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC9 (KO) knockout samples were subjected to SDS-PAGE. Ab109446 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.


    • All lanes : Anti-HDAC9 antibody [EPR5223] (ab109446) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 111 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    • ab109446 at 1/250 dilution staining HDAC9 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109446 at 1/100 dilution staining HDAC9 in HeLa cells by Immunofluorescence.

    References for Anti-HDAC9 antibody [EPR5223] (ab109446)

    This product has been referenced in:
    • Di Giorgio E  et al. The co-existence of transcriptional activator and transcriptional repressor MEF2 complexes influences tumor aggressiveness. PLoS Genet 13:e1006752 (2017). WB, ChIP ; Human . Read more (PubMed: 28419090) »
    • Sebastian S  et al. Tissue-specific splicing of a ubiquitously expressed transcription factor is essential for muscle differentiation. Genes Dev 27:1247-59 (2013). Read more (PubMed: 23723416) »

    See all 3 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Basilar Artery)
    Permeabilization
    Yes - 0.1% Triton-X PBS
    Specification
    Basilar Artery
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Username

    Courtney Fisher

    Verified customer

    Submitted Apr 19 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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