Overview

Description

  • Nature
    Synthetic

Specifications

Our Abpromise guarantee covers the use of ab41711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

    Western blot

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS, pH 7.2

General Info

  • Alternative names
    • HD 7
    • HD 7B
    • HD 9
    • HD7
    • HD7B
    • HD9
    • HDAC
    • HDAC 7
    • HDAC 7B
    • HDAC 9
    • HDAC 9B
    • HDAC 9FL
    • HDAC7
    • HDAC7B
    • HDAC9
    • HDAC9_HUMAN
    • HDAC9B
    • HDAC9FL
    • HDRP
    • Histone deacetylase 4/5 related protein
    • Histone deacetylase 7
    • Histone deacetylase 7B
    • Histone deacetylase 9
    • Histone deacetylase 9A
    • Histone deacetylase related protein
    • Histone deacetylase-related protein
    • KIAA0744
    • MEF2 interacting transcription repressor MITR
    • MEF2 interacting transcription repressor protein
    • MEF2-interacting transcription repressor MITR
    • MITR
    see all
  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
    Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
  • Tissue specificity
    Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
  • Involvement in disease
    Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Post-translational
    modifications
    Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Sumoylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt

References

ab41711 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

I'd suggest trying to use the peptide in excess and incubating the peptide longer with the antibody as well as increasing the incubation volume.

Thank you for contacting us.

Blocking will be inefficient if you are mixing the antibody and peptide after first diluting to the working solutions. The better approach is to mix the peptide and antibody stock solutions, then dilute to the wo...

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