Overview

  • Product name
  • Description
    Rabbit polyclonal to HDC2 / PHC2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 553-835 of Human HDC2/ PHC2 (BC029269; UniProt ID: Q8IXK0).

  • Positive control
    • HEK293 and MCF7 lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab124405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 36, 30 kDa (predicted molecular weight: 91,88,36,30 kDa).

Target

  • Function
    Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • Sequence similarities
    Contains 1 FCS-type zinc finger.
    Contains 1 SAM (sterile alpha motif) domain.
  • Domain
    HD1 motif interacts with SAM domain of PHC1.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    There are 5 isoforms produced by alternative splicing.
  • Alternative names
    • EDR2 antibody
    • early development regulator 2 (homolog of polyhomeotic 2) antibody
    • early development regulator 2 like antibody
    • Early development regulatory protein 2 antibody
    • hPH2 antibody
    • MGC163502 antibody
    • mph2 antibody
    • PH2 antibody
    • PHC2 antibody
    • PHC2_HUMAN antibody
    • polyhomeotic 2 antibody
    • polyhomeotic like 2 antibody
    • Polyhomeotic-like protein 2 antibody
    see all

Anti-HDC2 / PHC2 antibody images

  • All lanes : Anti-HDC2 / PHC2 antibody (ab124405) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : MCF7 cell lysate


    Predicted band size : 91,88,36,30 kDa
    Observed band size : 30,36 kDa (why is the actual band size different from the predicted?)

References for Anti-HDC2 / PHC2 antibody (ab124405)

ab124405 has not yet been referenced specifically in any publications.

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Treatment
2i and LIF
Specification
Mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
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Submitted Sep 15 2015

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