Overview

  • Product name
    Anti-HDGF antibody [26C4a]
    See all HDGF primary antibodies
  • Description
    Mouse monoclonal [26C4a] to HDGF
  • Tested applications
    Suitable for: WB, Dot blot, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of human HDGF, from an internal sequence.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab58644 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    26C4a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa).
Dot blot Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 21960592

Target

  • Function
    Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the HDGF family.
    Contains 1 PWWP domain.
  • Domain
    The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.
  • Post-translational
    modifications
    Sumoylated by SUMO1. Sumoylation prevents binding to chromatin.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ96580 antibody
    • HDGF antibody
    • HDGF_HUMAN antibody
    • Hepatoma derived growth factor antibody
    • Hepatoma-derived growth factor antibody
    • High mobility group protein 1 like 2 antibody
    • High mobility group protein 1-like 2 antibody
    • HMG-1L2 antibody
    • HMG1L2 antibody
    see all

Images

  • Anti-HDGF antibody [26C4a] (ab58644) at 1/100 dilution + immunising recombinant protein at 25 µg

    Secondary
    Sheep Anti Mouse IgG at 1/3000 dilution

    Predicted band size : 27 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Leich E  et al. MicroRNA profiles of t(14;18)-negative follicular lymphoma support a late germinal center B-cell phenotype. Blood 118:5550-8 (2011). IHC-P ; Human . Read more (PubMed: 21960592) »
  • Maschietto M  et al. Temporal blastemal cell gene expression analysis in the kidney reveals new Wnt and related signaling pathway genes to be essential for Wilms' tumor onset. Cell Death Dis 2:e224 (2011). Read more (PubMed: 22048167) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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