Validated using a knockout cell line
Recombinant
RabMAb

Anti-HDJ2 antibody [EPR7248] (ab126774)

Overview

  • Product name
    Anti-HDJ2 antibody [EPR7248]
    See all HDJ2 primary antibodies
  • Description
    Rabbit monoclonal [EPR7248] to HDJ2
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide, corresponding to residues near the C-terminus of Human HDJ2.

  • Positive control
    • HepG2, SKBR 3, Jurkat and Raji cell lysates; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 4.20 x 10 -11 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7248
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.
    • Sequence similarities
      Contains 1 CR-type zinc finger.
      Contains 1 J domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DJ 2 antibody
      • DJ2 antibody
      • DjA1 antibody
      • DnaJ (Hsp40) homolog, subfamily A, member 1 antibody
      • DnaJ homolog subfamily A member 1 antibody
      • DnaJ protein homolog 2 antibody
      • DNAJ2 antibody
      • Dnaja1 antibody
      • DNJA1_HUMAN antibody
      • hDJ 2 antibody
      • HDJ-2 antibody
      • HDJ2 antibody
      • Heat shock 40 kDa protein 4 antibody
      • heat shock protein DNAJ like 2 antibody
      • Heat shock protein J2 antibody
      • HSDJ antibody
      • HSJ-2 antibody
      • HSJ2 antibody
      • HSPF4 antibody
      • Human DnaJ protein 2 antibody
      • NEDD7 antibody
      • Neural precursor cell expressed developmentally down regulated 7 antibody
      • OTTHUMP00000021193 antibody
      see all

    Images



    • Predicted band size : 45 kDa

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)              
      Lane 2: HDJ2 knockout  HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)
      Lane 4: Jurkat whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab126774 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab126774 was shown to recognize HDJ2 in wild-type HAP1 samples along with additional cross-reactive bands. No band was observed when HDJ2 knockout samples were examined. Wild-type and HDJ2 knockout samples were subjected to SDS-PAGE.  Ab126774 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • ab126774 at 1/10 dilution staining HDJ2 in permeabilized Jurkat cells by Flow cytometry (red). Rabbit IgG negative control (green).
    • All lanes : Anti-HDJ2 antibody [EPR7248] (ab126774) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SKBR 3 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 45 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126774 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab126774.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up