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Abcam's HDL and LDL/VLDL Cholesterol Quantification Kit provides a simple quantification method of HDL and LDL/VLDL after a convenient separation of HDL from LDL and VLDL (very low-density lipoprotein) in serum samples. In the assay, cholesterol oxidase specifically recognizes free cholesterol and produces products which react with probe to generate color (570 nm) and fluorescence (Ex/Em = 538/587 nm). Cholesterol esterase hydrolizes cholesteryl ester into free cholesterol, therefore, cholesterol ester and free cholesterol can be detected separately in the presence and absence of cholesterol esterase in the reactions.
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Regulation of HDL (high-density-lipoprotein)-cholesterol and LDL (low-density-lipoprotein)-cholesterol plays a central role in various disease developments. It is well known that low levels of HDL and high level of LDL are associated with an increased risk of cardiovascular events.
If you need to purchase additional HDL precipitation buffer, please see ab105138.
|2X LDL/VLDL Precipitation Buffer||NW||1 x 10ml|
|Cholesterol Assay Buffer||WM,||1 x 25ml|
|Cholesterol Esterase (Lyophilized)||Blue||1 vial|
|Cholesterol Probe (in DMSO, anhydrous)||Red||1 x 200µl|
|Cholesterol Standard (2 ug/ul)||Yellow||1 x 100µl|
|Enzyme Mix (Lyophilized)||Green||1 vial|
Our Abpromise guarantee covers the use of ab65390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Serum HDL (top) and LDL/VLDL cholesterol (bottom) from 5-7 mice/group was measured using ab65390 following protocol instructions. An asterisk (*) means P<0.05 between WT ad FXR-KO vehicle group. A pound (#) indicates P<0.05 between WT and FXR-KO HFD group.Image obtained from Li G et al., PLoS One, 2012; 7(4): e35895
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"