Recombinant Anti-HDLBP antibody [EPR5268] (ab133594)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5268] to HDLBP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HDLBP antibody [EPR5268]
See all HDLBP primary antibodies -
Description
Rabbit monoclonal [EPR5268] to HDLBP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, 293T, Jurkat, HeLa, RAW 264.7, PC12 and NIH 3T3 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.60 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5268 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 155 kDa (predicted molecular weight: 141 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 155 kDa (predicted molecular weight: 141 kDa). |
Target
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Function
Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol. -
Sequence similarities
Contains 14 KH domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3069 Human
- Entrez Gene: 110611 Mouse
- Entrez Gene: 64474 Rat
- Omim: 142695 Human
- SwissProt: Q00341 Human
- SwissProt: Q8VDJ3 Mouse
- SwissProt: Q9Z1A6 Rat
- Unigene: 471851 Human
see all -
Alternative names
- HBP antibody
- HDL binding protein antibody
- HDL-binding protein antibody
see all
Images
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All lanes : Anti-HDLBP antibody [EPR5268] (ab133594) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 141 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133594 has not yet been referenced specifically in any publications.