Overview

  • Product name
  • Description
    Rabbit polyclonal to HE4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human mature HE4 fused to glutathione S-transferase. Produced in E.coli.

  • Positive control
    • Normal human epididymis or human ovarian tumour.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 25 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
  • Sequence similarities
    Contains 2 WAP domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Form
    There are 5 isoforms produced by alternative splicing.
  • Alternative names
    • dJ461P17.6 antibody
    • EDDM4 antibody
    • epididymal protein 4 antibody
    • Epididymal secretory protein E4 antibody
    • Epididymis specific whey acidic protein type four disulfide core antibody
    • HE 4 antibody
    • Major epididymis specific protein E4 antibody
    • Major epididymis-specific protein E4 antibody
    • MGC57529 antibody
    • Putative protease inhibitor WAP5 antibody
    • WAP 5 antibody
    • WAP domain containing protein HE4 antibody
    • WAP domain containing protein HE4 V4 antibody
    • WAP four disulfide core domain 2 antibody
    • WAP four disulfide core domain protein 2 antibody
    • WAP four-disulfide core domain protein 2 antibody
    • WAP5 antibody
    • WFDC 2 antibody
    • WFDC2 antibody
    • WFDC2_HUMAN antibody
    see all

Images

  • ab24480 (4µg/ml) staining HE4 in human lung using an automated system (DAKO Autostainer Plus).
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:
  • Markovic-Lipkovski J  et al. Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis. PLoS One 10:e0137028 (2015). IHC-P ; Human . Read more (PubMed: 26327314) »
  • Tokuishi K  et al. Splice variant HE4-V3 expression is associated with favorable prognosis in pulmonary adenocarcinoma. Tumour Biol 33:103-9 (2012). WB, IHC-P ; Human . Read more (PubMed: 22015929) »

See all 5 Publications for this product

Customer reviews and Q&As

The antibody is a polyclonal that was raised against a GST-fusion of the full length HE4 protein. As a result, it is likely to recognize all isoforms, but this has not been specifically tested.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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