Overview

  • Product name
  • Description
    Rabbit polyclonal to HE4
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human HE4.

    (Peptide available as ab98946.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human lung; Human ovary.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 13 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Tissue specificity
    Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
  • Sequence similarities
    Contains 2 WAP domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Form
    There are 5 isoforms produced by alternative splicing.
  • Alternative names
    • dJ461P17.6 antibody
    • EDDM4 antibody
    • epididymal protein 4 antibody
    • Epididymal secretory protein E4 antibody
    • Epididymis specific whey acidic protein type four disulfide core antibody
    • HE 4 antibody
    • Major epididymis specific protein E4 antibody
    • Major epididymis-specific protein E4 antibody
    • MGC57529 antibody
    • Putative protease inhibitor WAP5 antibody
    • WAP 5 antibody
    • WAP domain containing protein HE4 antibody
    • WAP domain containing protein HE4 V4 antibody
    • WAP four disulfide core domain 2 antibody
    • WAP four disulfide core domain protein 2 antibody
    • WAP four-disulfide core domain protein 2 antibody
    • WAP5 antibody
    • WFDC 2 antibody
    • WFDC2 antibody
    • WFDC2_HUMAN antibody
    see all

Images

  • All lanes : Anti-HE4 antibody (ab85179) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 13 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)


    Exposure time : 16 minutes
  • ICC/IF image of ab85179 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85179, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.

References

This product has been referenced in:
  • Markovic-Lipkovski J  et al. Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis. PLoS One 10:e0137028 (2015). ICC/IF ; Human . Read more (PubMed: 26327314) »
  • Eade CR  et al. HIV-Enhancing Factors Are Secreted by Reproductive Epithelia upon Inoculation with Bacterial Vaginosis-Associated Bacteria. Protein Pept Lett 22:672-80 (2015). Read more (PubMed: 25751267) »

See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (15 % Tris-HCl criterion precast gel (Biorad))
Sample
Horse Cell lysate - other (equine concentrated oviductal flush)
Specification
equine concentrated oviductal flush
Treatment
concentrated (centrivap) + resuspended
Blocking step
Tween-20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.3% · Temperature: 18°C
Username

Ms. Hilde Nelis

Verified customer

Submitted Oct 25 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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