Overview

  • Product nameAnti-HEAB antibody
    See all HEAB primary antibodies
  • Description
    Rabbit polyclonal to HEAB
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within the internal sequence amino acids 142-341 of Human HEAB.

  • Positive control
    • A549, HeLa, HepG2, HCT116 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab96309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.

Target

  • FunctionPolynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
  • Sequence similaritiesBelongs to the Clp1 family. Clp1 subfamily.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP/GTP binding protein antibody
    • Cleavage and polyadenylation factor I subunit 1 antibody
    • CLP 1 antibody
    • clp1 antibody
    • CLP1 cleavage and polyadenylation factor I subunit homolog antibody
    • CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) antibody
    • CLP1, yeast, homolog of antibody
    • CLP1_HUMAN antibody
    • hClp1 antibody
    • Homolog of yeast CFIA subunit Clp1p antibody
    • Polyadenylation factor Clp1 antibody
    • Polynucleotide kinase Clp1 antibody
    • Polyribonucleotide 5' hydroxyl kinase Clp1 antibody
    • Polyribonucleotide 5''-hydroxyl-kinase Clp1 antibody
    • Pre mRNA cleavage complex II protein Clp1 antibody
    • Pre-mRNA cleavage complex II protein Clp1 antibody
    see all

Anti-HEAB antibody images

  • All lanes : Anti-HEAB antibody (ab96309) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa

References for Anti-HEAB antibody (ab96309)

ab96309 has not yet been referenced specifically in any publications.

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