Heat Mediated High pH Antigen Retrieving Solution (ab972)

Overview

  • Product nameHeat Mediated High pH Antigen Retrieving Solution
    See all Antigen retrieval solution reagents
  • Tested applicationsSuitable for: IHC-Pmore details
  • General notesThis antigen retrieval solution (pH 9.5) incorporates a colored pH indicator. The end-user is assured, by visual inspection, that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperatures, and will help prevent the possibility of losing pH sensitive antigens. When heating, the solution should turn gray. If the color of the solution turns yellow or red, it is an indication that the pH of the solution is incorrect and that staining may be hindered.


    This one-step high pH solution (9.5) allows the end-user to simultaneously deparaffinize and to heat-pretreat formalin-fixed paraffin-embedded tissues. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reduce costs for reagents and hazardous waste. Alternatively, tissues can be deparaffinized in xylene and hydrated to water, and then placed into the solution in the pressure cooker and/or other heating methods. This product also has blocking reagents to reduced non-specific background staining.

Properties

  • FormLiquid
  • Storage instructionsStore at room temperature.
  • Storage bufferpH: 9.50
    Preservative: Proprietary preservative
    Constituents: Carrier protein, PBS
    Note: Proprietary preservative
  • Concentration information loading...
  • Reagent notesThis one-step high pH solution (9.5) allows the end-user to simultaneously deparaffinize and to heat-pretreat formalin-fixed paraffin-embedded tissues. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reduce costs for reagents and hazardous waste. Alternatively, tissues can be deparaffinized in xylene and hydrated to water, and then placed into the solution in the pressure cooker and/or other heating methods. This product also has blocking reagents to reduced non-specific background staining.
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab972 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use at an assay dependent concentration.

    References for Heat Mediated High pH Antigen Retrieving Solution (ab972)

    This product has been referenced in:
    • Rosen D  et al. Accelerated thymic maturation and autoreactive T cells in bronchopulmonary dysplasia. Am J Respir Crit Care Med 174:75-83 (2006). IHC-P ; Baboon . Read more (PubMed: 16574933) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. Our QC procedure is to heat the Borg up to 125 degrees for 30 second and then cool down to 90 degrees. This procedure takes place in our pressure cooker. The entire process takes 35 minutes. We have not tried it in an autocl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"