Anti-Heat Shock Factor 2 Binding Protein antibody (ab126252)

Overview

  • Product name
    Anti-Heat Shock Factor 2 Binding Protein antibody
    See all Heat Shock Factor 2 Binding Protein primary antibodies
  • Description
    Rabbit polyclonal to Heat Shock Factor 2 Binding Protein
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1 -219 of the Human Heat Shock Factor 2 Binding Protein (O75031).

  • Positive control
    • NT2D1 and SK-N-SH cell lysates, Human U87 xenograft and Mouse Muscle tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    Heat Shock Factor 2 Binding Protein (HSF2BP) associates with Heat Shock Factor 2 (HSF2). The interaction occurs between the trimerization domain of HSF2 and the amino terminal hydrophilic region of HSF2BP that comprises two leucine zipper motifs. HSF2BP may therefore be involved in modulating HSF2 activation.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • heat shock transcription factor 2 binding protein antibody
    • HSF2 binding protein antibody
    • HSF2BP antibody

Images

  • ab126252, at 1/500, staining Heat Shock Factor 2 Binding Protein in Human U87 xenograft by immunohistochemistry [Paraffin Embedded Tissues (IHC-P).
  • ab126252, at 1/500, staining Heat Shock Factor 2 Binding Protein in Mouse Muscle by immunohistochemistry [Paraffin Embedded Tissues (IHC-P).
  • All lanes : Anti-Heat Shock Factor 2 Binding Protein antibody (ab126252) at 1/1000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : SK-N-SH whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 38 kDa

References

ab126252 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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