![Immunofluorescence - heavy chain Myosin antibody [H11] (ab24642) image](#ab24642_1.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
Overview
Product name
Anti-heavy chain Myosin antibody [H11]
See all heavy chain Myosin products (5) ...
Description
Mouse monoclonal [H11] to heavy chain Myosin
Tested applications
IF, WB, IPmore details
Cross reactivity
Reacts with
Rat
Predicted to work with
all Mammals
Does not react with
Amphibians
Immunogen
Complex mixture of phosphoproteins extracted from Madin-Darby Canine Kidney Cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Primary antibody notes
This antibody binds specifically to denatured as well as native myosin heavy chain extracts.
Clonality
Monoclonal
Clone number
H11
Isotype
IgM
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
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Immunofluorescence - heavy chain Myosin antibody [H11] (ab24642)
![Immunofluorescence - heavy chain Myosin antibody [H11] (ab24642) image](/ps/datasheet/Images/24/ab24642/ab24642_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab24642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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IF: 1/500
WB: 1/1000Predicted molecular weight: 224 kDa.
IP: 1/150(Recommend using extracts prepared in buffers containing 0.5% Triton X-100 and 0.5% sodium deoxycholate.)
Target
Function
Muscle contraction.
Involvement in disease
Defects in MYH3 are the cause of distal arthrogryposis type 2A (DA2A) [MIM:193700]; also known as Freeman-Sheldon syndrome (FSS). Distal arthrogryposis is a clinically and genetically heterogeneous group of disorders characterized by bone anomalies and joint contractures of the hands and feet, causing medially overlapping fingers, clenched fists, ulnar deviation of fingers, camptodactyly and positional foot deformities. It is a disorder of primary limb malformation without primary neurologic or muscle disease. DA2A is the most severe form of distal arthrogryposis. Affected individuals have contractures of the orofacial muscles, characterized by microstomia with pouting lips, H-shaped dimpling of the chin, deep nasolabial folds, and blepharophimosis. Dysphagia, failure to thrive, growth deficit, and life-threatening respiratory complications (caused by structural anomalies of the oropharynx and upper airways) are frequent. Inheritance is autosomal dominant.
Defects in MYH3 are the cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as Sheldon-Hall syndrome (SHS) or arthrogryposis multiplex congenita distal type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. DA2B is the most common of the distal arthrogryposis syndromes. It is similar to DA2A but the facial contractures are less dramatic.
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain.
Developmental stage
Abundantly present in fetal skeletal muscle and not present or barely detectable in heart and adult skeletal muscle.
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Cellular localization
Cytoplasm > myofibril. Thick filaments of the myofibrils.
Target information above from: UniProt accessionP11055
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- embryonic antibody
- fast skeletal muscle antibody
- HEMHC antibody
see all
Database links
Anti-heavy chain Myosin antibody [H11] images:
Immunofluorescence - heavy chain Myosin antibody [H11] (ab24642)
ab24642 staining of myosin fibers. Actomyosin cytoskeletal fibers have a 'beads on string' appearance.
References for Anti-heavy chain Myosin antibody [H11] (ab24642)
This product has been referenced in:
- Martini Cet al. 3-hydroxy 3-methylglutaryl coenzyme A reductase increase is essential for rat muscle differentiation. J Cell Physiol 220:524-30 (2009). WB; Rat.Read more (PubMed: 19388010) »
See 1 publication for this product
Publishing research using ab24642? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"