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Product Name
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heavy chain Myosin antibody [WB-MHCs]
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See all heavy chain Myosin antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [WB-MHCs] to heavy chain Myosin
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Immunogen
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Native myosin extracted from rabbit soleus muscle.
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Reacts with
(species key)
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Hu, Ms, Rat, Dog, Goat, Pig, Rb, Shp
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Specificity
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This antibody is specific to heavy chain Myosin (myosin slow type heavy chain).
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Tested applications
(see key)
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IHC-Fr
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fr (unfixed): 1/20 - 1/80.
Is unsuitable for IHC-P or WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Immunohistochemistry: Type I myosin heavy chain in human, rat, mouse, rabbit, dog, sheep, pig and goat muscle.
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Cellular localization
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Thick filaments of the myofibrils.
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
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Relevance
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Heavy chain myosin functions in muscle contraction. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC2). Abundantly present in fetal skeletal muscle and not present or barely detectable in heart and adult skeletal muscle. The rod-like tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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WB-MHCs
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Myeloma
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P3-NS1/1-Ag4-1
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Isotype
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IgG1
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this heavy chain Myosin antibody [WB-MHCs] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to heavy chain Myosin
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heavy chain Myosin antibody [WB-MHCs] - more information
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