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Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [3-48] (ab15)
![Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [3-48] (ab15)](/ps/datasheet/images/ab15_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [3-48] (ab15)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [3-48] (ab15)](/ps/datasheet/images/ab15_2.jpg)
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Product Name
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heavy chain cardiac Myosin antibody [3-48]
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See all heavy chain cardiac Myosin antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [3-48] to heavy chain cardiac Myosin
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Immunogen
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Full length native protein (purified) (Human).
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Dog, Pig, Rb
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Specificity
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This antibody is myosin alpha and beta heavy chain specific.
Affinity constants for:
Human ventricular myosin heavy chains - 3.33 x 108M-1
Human atrial myosin heavy chains - 1.48 x 108M-1
Human skeletal muscle myosin - 1.06 x 108M-1
Canine cardiac myosin heavy chains - 2.06 x 108M-1
3-48 antibody cross-reacts with heavy chains of human myosin isolated from: slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide.
It does not react with human platelet, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells.
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Tested applications
(see key)
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ELISA, Flow Cyt, ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependant dilution
ICC/IF: Use at an assay dependent dilution (indirect immunofluorescence for human/canine myocytes).
Flow Cyt: Use at an assay dependent dilution PMID 17620508.
IHC (Carnoy fixed tissues): Use at an assay dependent dilution (indirect immunoperoxidase).
IHC-Fr: Use at an assay dependant dilution and amplification (indirect IF on frozen sections).
WB: Use at an assay dependant dilution. Predicted molecular weight: 223 kDa. According to SDS-PAGE under mercaptoethanol (reduced conditions) 153.8 kd
heavy chain: 49.7 kd light chain: 27.2 kd
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution (from PubMed: 16357009).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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slow skeletal muscle, fast skeletal muscle, eye muscle
ICC: human and canine cardiac myocytes
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Cellular localization
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Thick filaments of the myofibrils.
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Research areas
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Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Immunology >> Immune System Diseases >> Autoimmune
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
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Relevance
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Cardiac MHC exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the MHC genes are associated with several different dilated and hypertrophic cardiomyopathies.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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3-48
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Myeloma
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P3x63-Ag8.653
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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IgG fraction
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Storage buffer
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Phosphate buffered saline
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Purification notes
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Clinical grade tissue culture supernatant was concentrated and purified to homogeneity by a series of chromatographic steps.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this heavy chain cardiac Myosin antibody [3-48] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for heavy chain cardiac Myosin antibody [3-48] images, references, products related to ab15 and other tools.
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heavy chain cardiac Myosin antibody [3-48] images:
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 Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [3-48] (ab15)
ab15 at 1/100 dilution staining neonatal rat cardiomyocytes by ICC/IF. The cells were paraformaldehyde fixed and blocked in 5% serum prior to incubation with the antibody for 30 minutes. Cells were sequentially incubated with phalloidin-TRITC (to label actin, red), heavy chain cardiac Myosin primary antibody [ab15], goat anti-mouse FITC conjugated secondary antibody (green) and finally DNA was labelled with DAPI (blue). Cells were mounted in glycerol and imaged with fluorescence microscopy. This image is courtesy of an Abreview submitted by Dr Andrew Pelling
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [3-48] (ab15)
ab15 at 1/100 staining rat heart tissue sections by IHC-P. The tissue was fixed with Histochoice for 72 hr, paraffin sectioned and then the slide was baked prior to staining with ab15 overnight at 4C. The image depicts a bundle of heavy chain cardiac myosin expressing cardic myocytes (red) of the heart tissue and counterstained nuclei (DAPI-blue). Note, the oval nuclei are centrally located within the cardiac myocyte cell bodies. The lines that are perpendicular to the long axis of the fibers (cross striations) are made of alternate red and black zone. The red (A-bands) striated areas are where myosin filaments align and the black gap (unstained area; I-bands) in between red zone are where actin filaments align. This image is courtesy of an Abreview submitted by Dr Mal Niladri
See Abreview
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References for heavy chain cardiac Myosin antibody [3-48] (ab15)
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This product has been used in: (two most recent references)
Qiu Z et al. c-Abl tyrosine kinase regulates cardiac growth and development. Proc Natl Acad Sci U S A 107:1136-41 (2010). ICC/IF; Mouse. PubMed: 20080568
Taniguchi K et al. Mammalian formin fhod3 regulates actin assembly and sarcomere organization in striated muscles. J Biol Chem 284:29873-81 (2009). WB; Rat. PubMed: 19706596
See all 14 publication references for this product.
If you publish research using ab15 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to heavy chain cardiac Myosin
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heavy chain cardiac Myosin antibody [3-48] - more information
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heavy chain cardiac Myosin antibody [3-48] - related products:
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heavy chain cardiac Myosin antibody [3-48] - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [3-48] (ab15)](/index.html?datasheet=15#ab15_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [3-48] (ab15)](/index.html?datasheet=15#ab15_2.jpg)
