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Anti-heavy chain cardiac Myosin antibody [3-48] (ab15)

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Overview

Product name

Anti-heavy chain cardiac Myosin antibody [3-48]
See all heavy chain cardiac Myosin products (2) ...

Description

Mouse monoclonal [3-48] to heavy chain cardiac Myosin

Specificity

This antibody is myosin alpha and beta heavy chain specific. Affinity constants for: Human ventricular myosin heavy chains - 3.33 x 108M-1 Human atrial myosin heavy chains - 1.48 x 108M-1 Human skeletal muscle myosin - 1.06 x 108M-1 Canine cardiac myosin heavy chains - 2.06 x 108M-1 3-48 antibody cross-reacts with heavy chains of human myosin isolated from: slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelet, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells.

Tested applications

IHC (PFA fixed), Flow Cyt, ELISA, IHC-Fr, WB, ICC, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Cow, Dog, Human, Pig

Immunogen

Full length native protein (purified) (Human).

Positive control

slow skeletal muscle, fast skeletal muscle, eye muscle ICC: human and canine cardiac myocytes

General notes

Molecular weight if IgG: according to SDS-PAGE under mercaptoethanol (reduced conditions) 153.8 kd. Heavy chain: 49.7 kd light chain: 27.2 kd. Molecular weight of F(ab')2: according to SDS-PAGE under mercaptoethanol (reduced conditions) 116 kd. Heavy chain: 30.8 kd light chain: 27.2 kd

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Clinical grade tissue culture supernatant was concentrated and purified to homogeneity by a series of chromatographic steps.

Clonality

Monoclonal

Clone number

3-48

Myeloma

P3x63-Ag8.653

Isotype

IgG1

Light chain type

kappa

Research areas

Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Immunology >> Immune System Diseases >> Autoimmune
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin

  • Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [3-48] (ab15)Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [3-48] (ab15) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [3-48] (ab15)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [3-48] (ab15) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab15 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Cardiac MHC exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the MHC genes are associated with several different dilated and hypertrophic cardiomyopathies.

Cellular localization

Thick filaments of the myofibrils.

Alternative names

  • Alpha MHC antibody
  • ASD3 antibody
  • CMD1S antibody
  • CMH1 antibody
  • MGC138376 antibody
  • MGC138378 antibody
  • MPD1 antibody
  • MYH 6 antibody
  • MYH 7 antibody
  • MYH6 antibody
  • MYH7 antibody
  • MYHC A antibody
  • MYHC antibody
  • MYHC B antibody
  • MyHC-alpha antibody
  • MyHC-beta antibody
  • MYHCA antibody
  • MYHCB antibody
  • Myosin heavy chain cardiac muscle alpha isoform antibody
  • Myosin heavy chain cardiac muscle beta isoform antibody
  • Myosin heavy polypeptide 7 cardiac muscle beta antibody
see all

Database links

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Anti-heavy chain cardiac Myosin antibody [3-48] images:

  Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [3-48] (ab15)

Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [3-48] (ab15)

ab15 at 1/100 dilution staining neonatal rat cardiomyocytes by ICC/IF. The cells were paraformaldehyde fixed and blocked in 5% serum prior to incubation with the antibody for 30 minutes. Cells were sequentially incubated with phalloidin-TRITC (to label actin, red), heavy chain cardiac Myosin primary antibody [ab15], goat anti-mouse FITC conjugated secondary antibody (green) and finally DNA was labelled with DAPI (blue). Cells were mounted in glycerol and imaged with fluorescence microscopy.

This image is courtesy of an Abreview submitted by Dr Andrew Pelling

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [3-48] (ab15)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [3-48] (ab15)

ab15 at 1/100 staining rat heart tissue sections by IHC-P. The tissue was fixed with Histochoice for 72 hr, paraffin sectioned and then the slide was baked prior to staining with ab15 overnight at 4C. The image depicts a bundle of heavy chain cardiac myosin expressing cardic myocytes (red) of the heart tissue and counterstained nuclei (DAPI-blue). Note, the oval nuclei are centrally located within the cardiac myocyte cell bodies. The lines that are perpendicular to the long axis of the fibers (cross striations) are made of alternate red and black zone. The red (A-bands) striated areas are where myosin filaments align and the black gap (unstained area; I-bands) in between red zone are where actin filaments align.

This image is courtesy of an Abreview submitted by Dr Mal Niladri

See Abreview

References for Anti-heavy chain cardiac Myosin antibody [3-48] (ab15)

This product has been referenced in:

  • Haghikia Aet al. Signal transducer and activator of transcription 3-mediated regulation of miR-199a-5p links cardiomyocyte and endothelial cell function in the heart: a key role for ubiquitin-conjugating enzymes. Eur Heart J 32:1287-97 (2011).Read more (PubMed: 20965886) »
  • Asumda FZ & Chase PB Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol 12:44 (2011).Read more (PubMed: 21992089) »

See all 21 publications for this product

Publishing research using ab15? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"