Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
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ab50967 has been referenced in 20 publications.
Publishing research using ab50967? Please let us know so that we can cite the reference in this datasheet
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ab50967 at a 1/200 dilution staining heavy chain cardiac Myosin in murine heterozygote cardiomyocytes by Immunocytochemistry/ Immunofluorescence.
Image from Mayers CM et al, J Biol Chem. 2010 Apr 16;285(16):12344-54. Epub 2010 Feb 5, Fig 3.
Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 223 kDa
Observed band size : 223 kDa
ab50967 staining mouse heart tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer pH 6, prior to blocking with sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd
All lanes : Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 223 kDa
Observed band size : 223 kDa
Additional bands at : 48 kDa,95 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
ab50967 staining heavy chain cardiac Myosin in murine HL-1 cells (derived from an atrial tumor) by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with 10% serum for 30 minutes at 21°C and then incubated with ab50967 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a goat anti-mouse polyclonal conjugated to Alexa-Fluor 488, used at a 1/400 dilution. Counterstained with DAPI.
Image courtesy of an anonymous Abreview.
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