You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-heavy chain cardiac Myosin antibody [BA-G5]
See all heavy chain cardiac Myosin products (2) ...

Description

Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin

Specificity

Although this antibody was raised against myosin from skeletal muscle in cows, it has been shown to be specific for heavy chain cardiac myosin in other species (see references). It has been reported previously that BA-G5 is specific for the alpha isoform of heavy chain cardiac myosin, however other reports suggest that it also reacts with the beta isoform (PubmedID: 10412087).

Tested applications

WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Human

Predicted to work with

Cow

Immunogen

Cow skeletal muscle myosin

Positive control

This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

BA-G5

Isotype

IgG2b

Research areas

Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Immunology >> Immune System Diseases >> Autoimmune
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin

  • Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [BA-G5] (ab50967) image (enlarge)

  • Western blot - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)Western blot - heavy chain cardiac Myosin antibody [BA-G5] (ab50967) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [BA-G5] (ab50967) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Cardiac MHC exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the MHC genes are associated with several different dilated and hypertrophic cardiomyopathies.

Cellular localization

Thick filaments of the myofibrils.

Alternative names

  • Alpha MHC antibody
  • ASD3 antibody
  • CMD1S antibody
  • CMH1 antibody
  • MGC138376 antibody
  • MGC138378 antibody
  • MPD1 antibody
  • MYH 6 antibody
  • MYH 7 antibody
  • MYH6 antibody
  • MYH7 antibody
  • MYHC A antibody
  • MYHC antibody
  • MYHC B antibody
  • MyHC-alpha antibody
  • MyHC-beta antibody
  • MYHCA antibody
  • MYHCB antibody
  • Myosin heavy chain cardiac muscle alpha isoform antibody
  • Myosin heavy chain cardiac muscle beta isoform antibody
  • Myosin heavy polypeptide 7 cardiac muscle beta antibody
see all

Database links

see all

Anti-heavy chain cardiac Myosin antibody [BA-G5] images:

  Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

Immunocytochemistry/ Immunofluorescence - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

ab50967 at a 1/200 dilution staining heavy chain cardiac Myosin in murine heterozygote cardiomyocytes by Immunocytochemistry/ Immunofluorescence.

Image from Mayers CM et al, J Biol Chem. 2010 Apr 16;285(16):12344-54. Epub 2010 Feb 5, Fig 3.

  Western blot - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

Western blot - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg

Secondary
IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 223 kDa
Observed band size : 223 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

ab50967 staining mouse heart tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer pH 6, prior to blocking with sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C.  A HRP conjugated goat anti-mouse antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Antibody Solutions Ltd

See Abreview

  Western blot - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

Western blot - heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

All lanes : Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 1 µg/ml

Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 223 kDa
Observed band size : 223 kDa
Additional bands at : 48 kDa,95 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 4 minutes

  Immunocytochemistry/ Immunofluorescence - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

Immunocytochemistry/ Immunofluorescence - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

ab50967 staining heavy chain cardiac Myosin in murine HL-1 cells (derived from an atrial tumor) by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with 10% serum for 30 minutes at 21°C and then incubated with ab50967 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a goat anti-mouse polyclonal conjugated to Alexa-Fluor 488, used at a 1/400 dilution. Counterstained with DAPI.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

This product has been referenced in:

  • Wang Jet al. Expression of unique and developmental myosin heavy chain isoforms in adult human digastric muscle. J Histochem Cytochem 52:851-9 (2004). IHC-Fr; Human.Read more (PubMed: 15208352) »
  • Lucas CA & Hoh JF Distribution of developmental myosin heavy chains in adult rabbit extraocular muscle: identification of a novel embryonic isoform absent in fetal limb. Invest Ophthalmol Vis Sci 44:2450-6 (2003). IHC-Fr; Rabbit.Read more (PubMed: 12766042) »

See all 20 publications for this product

Publishing research using ab50967? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"