Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

Overview

  • Product name
    Anti-heavy chain cardiac Myosin antibody [BA-G5]
    See all heavy chain cardiac Myosin primary antibodies
  • Description
    Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin
  • Tested applications
    Suitable for: WB, IHC-P, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Cow
  • Immunogen

    Cow skeletal muscle myosin

  • Positive control
    • This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle
  • General notes

    Alternative versions available:

    Anti-heavy chain cardiac Myosin antibody [BA-G5] - BSA and Azide free (ab174640)

Properties

Applications

Our Abpromise guarantee covers the use of ab50967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P 1/100.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20139090

Target

Anti-heavy chain cardiac Myosin antibody [BA-G5] images

  • Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 223 kDa
  • ab50967 staining heavy chain cardiac Myosin in murine HL-1 cells (derived from an atrial tumor) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with 10% serum for 30 minutes at 21°C and then incubated with ab50967 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a goat anti-mouse polyclonal conjugated to Alexa-Fluor 488, used at a 1/400 dilution. Counterstained with DAPI.

    See Abreview

  • ab50967 staining mouse heart tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer pH 6, prior to blocking with sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C.  A HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • ab50967 at a 1/200 dilution staining heavy chain cardiac Myosin in murine heterozygote cardiomyocytes by Immunocytochemistry/ Immunofluorescence.
  • All lanes : Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 1 µg/ml

    Lane 1 : Heart (Rat) Tissue Lysate
    Lane 2 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 223 kDa
    Additional bands at : 48 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ab50967 staining heavy chain cardiac Myosin in Mouse whole embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in 5% Goat serum) for 16 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)

This product has been referenced in:
  • Zhang Y  et al. CRISPR-Cpf1 correction of muscular dystrophy mutations in human cardiomyocytes and mice. Sci Adv 3:e1602814 (2017). WB ; Human . Read more (PubMed: 28439558) »
  • Zhang Q  et al. Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation. Sci Rep 6:30146 (2016). WB . Read more (PubMed: 27443826) »

See all 27 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 27 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample
Rat Tissue sections (heart)
Specification
heart
Permeabilization
Yes - Triton x-100, 0.01%
Fixative
Formaldehyde
Username

Dr. Shyam Sundar Nandi

Verified customer

Submitted Nov 20 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse whole embryo)
Specification
mouse whole embryo
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Feb 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
50 µg
Specification
mouse heart
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Aug 29 2012

Vielen Dank für Ihre Antwortund dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit unseren Antikörpern hatten.

Um Ihr Problem zu lösen, m&o...

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Thank you for contacting us. Unfortunately, I would not recommend using ab66470 as a positive control in Western blot: It is a synthetic peptide derived from within residues 600 - 700 of Human beta-cardiac myosin heavy chain. This peptide was used a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HL-1 cells (derived from murine atrial tumor))
Specification
HL-1 cells (derived from murine atrial tumor)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Sep 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (heart smooth muscle)
Loading amount
30 µg
Specification
heart smooth muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cardiac)
Specification
cardiac
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (heart atria)
Loading amount
15 µg
Specification
heart atria
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Prof. Paul Townsend

Verified customer

Submitted Apr 21 2008

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