Cow skeletal muscle myosin
Alternative versions available:
Anti-heavy chain cardiac Myosin antibody [BA-G5] - BSA and Azide free (ab174640)
Our Abpromise guarantee covers the use of ab50967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).|
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20139090|
ab50967 staining mouse heart tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer pH 6, prior to blocking with sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.