Overview

  • Product name
  • Description
    Rabbit polyclonal to HEC1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human HEC1.
    Database link: O14777

  • Positive control
    • K562, LOVO, MCF7, 22RV-1, 293T and THP1 cell lysates; mouse testis lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab186839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 74 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • Sequence similarities
    Belongs to the NDC80/HEC1 family.
  • Developmental stage
    Expression peaks in mitosis.
  • Post-translational
    modifications
    Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • Database links
  • Alternative names
    • from Entrez Gene ; antibody
    • HEC antibody
    • Highly expressed in cancer antibody
    • Highly expressed in cancer protein antibody
    • Highly expressed in cancer rich in leucine heptad repeats antibody
    • HsHec1 antibody
    • hsNDC80 antibody
    • Kinetochore associated 2 antibody
    • Kinetochore associated protein 2 antibody
    • Kinetochore protein Hec1 antibody
    • Kinetochore protein NDC80 homolog antibody
    • Kinetochore-associated protein 2 antibody
    • KNTC2 antibody
    • ndc80 antibody
    • NDC80 homolog kinetochore complex component antibody
    • NDC80 kinetochore complex component homolog antibody
    • NDC80, S. cerevisiae, homolog of antibody
    • NDC80_HUMAN antibody
    • Retinoblastoma associated protein HEC antibody
    • Retinoblastoma-associated protein HEC antibody
    • TID3 antibody
    see all

Images

  • All lanes : Anti-HEC1 antibody (ab186839) at 1/500 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : LOVO cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : 22RV-1 cell lysate
    Lane 5 : 293T cell lysate
    Lane 6 : mouse testis lysate
    Lane 7 : THP1 cell lysate

    Predicted band size: 74 kDa

References

ab186839 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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