Anti-HEC1 antibody (ab3393)

Overview

  • Product nameAnti-HEC1 antibody
    See all HEC1 primary antibodies
  • Description
    Goat polyclonal to HEC1
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: YEKKATLIKSSEE, corresponding to C terminal amino acids 630-642 of Human HEC1.

  • Positive control
    • Hela lysate.
  • General notesGenBank Accession Number – NP_006092 Gene Ontology terms - mitotic chromosome segregation; mitosis; centromere; nucleus

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesPeptide ELISA: antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 74 kDa).

    This antibody does not seem suitable for using in ICC/IF (please refer to Abreview).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
    • Sequence similaritiesBelongs to the NDC80/HEC1 family.
    • Developmental stageExpression peaks in mitosis.
    • Post-translational
      modifications
      Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
    • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
    • Information by UniProt
    • Database links
    • Alternative names
      • from Entrez Gene ; antibody
      • HEC antibody
      • Highly expressed in cancer antibody
      • Highly expressed in cancer protein antibody
      • Highly expressed in cancer rich in leucine heptad repeats antibody
      • HsHec1 antibody
      • hsNDC80 antibody
      • Kinetochore associated 2 antibody
      • Kinetochore associated protein 2 antibody
      • Kinetochore protein Hec1 antibody
      • Kinetochore protein NDC80 homolog antibody
      • Kinetochore-associated protein 2 antibody
      • KNTC2 antibody
      • ndc80 antibody
      • NDC80 homolog kinetochore complex component antibody
      • NDC80 kinetochore complex component homolog antibody
      • NDC80, S. cerevisiae, homolog of antibody
      • NDC80_HUMAN antibody
      • Retinoblastoma associated protein HEC antibody
      • Retinoblastoma-associated protein HEC antibody
      • TID3 antibody
      see all

    Anti-HEC1 antibody images



    • Predicted band size : 74 kDa


      ab3393 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References for Anti-HEC1 antibody (ab3393)

    ab3393 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Dec 15 2006

    Thank you for your enquiry. To our knowledge, the antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, we encourage you to subm...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"